Browsing by Author "El Senousy A.S."

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Anticancer potentiality of lignan rich fraction of six Flaxseed cultivars
(Nature Publishing Group, 2018) Ezzat, Shahira M; Shouman S.A.; Elkhoely A.; Attia Y.M.; Elsesy M.S.; El Senousy A.S.; Choucry M.A.; El Gayed S.H.; El Sayed A.A.; Sattar E.A.; El Tanbouly N.; Pharmacognosy Department; Faculty of Pharmacy; Cairo University; Kasr El-Einy Street; Cairo; 11562; Egypt; Department of Pharmacognosy; Faculty of Pharmacy; October University for Modern Science and Arts (MSA); 6th October; 12566; Egypt; Cancer Biology Department; National Cancer Institute; Cairo University; Cairo; Egypt; Pharmacology and Toxicology Department; Faculty of Pharmacy; Helwan University; Helwan; Egypt
The objective of our study is to highlight the therapeutic effect and mechanism of action by which purified Flaxseed hydrolysate (PFH) which is a lignan rich fraction exerts its anticancer activity on a human breast cancer cell line (T47D) and in mice bearing tumor. HPLC analysis of PFH of six flaxseed cultivars had shown that PFH of the cultivar Giza 9 (PFH-G9) contains the highest concentration of SDG (81.64 mg/g). The in vitro cytotoxic potentiality of PFH's of six flaxseed cultivars was screened against a panel of human cancer cell lines. PFH -G9 showed the most significant cytotoxic activity against ER-receptor positive breast cell lines MCF7 and T47D with IC50 13.8 and 15.8 ?g/ml, respectively. Moreover, PFH-G9 reduced the expression of the metastasis marker, 1-?, metalloproteinases and vascular endothelial growth factor (VEGF), one of the most potent stimulators of angiogenesis, while it increased the caspase-3 dependent apoptosis. Our study also showed that dietary intake of 10% of Giza 9 Flaxseeds (FS), fixed oil (FSO) or Flax meal (FSM) twice daily for 3 weeks in mice-bearing solid Ehrlich ascites carcinoma (EAC) resulted in reducing the tumor volume, the expression of estrogen, insulin growth factor, progesterone, VEGF and MMP-2, but enhanced expression of caspase-3. � 2018 The Author(s).
Antidepressant-Like Effect of Selected Egyptian Cultivars of Flaxseed Oil on a Rodent Model of Postpartum Depression
(Hindawi Limited, 2017) El Tanbouly N.; El Sayed A.M.; Ali Z.Y.; Abdel Wahab S.; El Gayed S.H.; Ezzat, Shahira M; El Senousy A.S.; Choucry M.A.; Abdel-Sattar E.; Department of Pharmacognosy; College of Pharmacy; Cairo University; Cairo; 11562; Egypt; Department of Biochemistry; National Organization for Drug Control and Research; Giza; 12553; Egypt; Department of Pharmacognosy; College of Pharmacy; October University for Modern Sciences and Arts; 6th of October12566; Egypt
Flaxseed (Linum usitatissimum L.) is a multipurpose crop with health promoting potential. This study was undertaken to investigate the fatty acid profile and yield of fixed oil of six Egyptian flaxseed cultivars. The selected cultivars with the highest content of polyunsaturated fatty acids (PUFAs) (G9 and G10) were assessed for their antidepressant-like effect in rat model of postpartum depression (PPD) induced by hormone-simulated pregnancy followed by hormone withdrawal and compared to fluoxetine. As compared to control group, administration of G9 and G10 (270 mg/kg/day, p.o) for two weeks during the postpartum period can alleviate anxiety and depressive-like behaviors and biochemical changes in PPD-induced rats. This was confirmed by evaluation of anxiety-like behaviors (elevated plus maze, open field test, and forced swim test tests), in addition to biochemical analysis (brain monoamine oxidase-A, corticosterone level, proinflammatory cytokines, and hippocampal redox state). In conclusion, flaxseed oil of Egyptian cultivars G9 and G10 exhibited significant antidepressant-like effect in rat model of PPD without affecting locomotor activity. At the treatment doses, the antidepressant-like activity of Giza 9 oil is comparable to fluoxetine. � 2017 Nebal El Tanbouly et al.