Browsing by Author "El Kholy A."

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    Carbapenem-resistant Gram-negative bacteria associated with catheter-related bloodstream infections in three intensive care units in Egypt
    (IEEE Computer Society, 2018) Abdulall A.K.; Tawfick M.M.; El Manakhly A.R.; El Kholy A.; Microbiology and Immunology Department; Faculty of Pharmacy (Girls); Al-Azhar University; Cairo; Egypt; Microbiology and Immunology Department; Faculty of Pharmacy (Boys); Al-Azhar University; Cairo; Egypt; Microbiology and Immunology Department; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); 6th October City; Giza; Egypt; Infection Control Department; Dar Al Fouad Hospital; 6th October City; Giza; Egypt; Clinical Pathology Department; Faculty of Medicine; Cairo University; Cairo; Egypt
    We aimed to identify the carbapenem-resistant Gram-negative bacteria (GNB) causing catheter-related bloodstream infections (CRBSI) in intensive care units (ICU) in a tertiary care Egyptian hospital, to study their resistance mechanisms by phenotypic and genetic tests, and to use ERIC-PCR for assessing their relatedness. The study was conducted over 2�years in three ICUs in a tertiary care hospital in Egypt during 2015�2016. We identified 194 bloodstream infections (BSIs); 130 (67.01%) were caused by GNB, of which 57 were isolated from CRBSI patients (73.84%). Identification of isolates was performed using conventional methods and MALDI-TOF MS. Antimicrobial susceptibility testing (AST) was done by disc diffusion following CLSI guidelines. Phenotypic detection of carbapenemases enzymes activity was by modified Hodge test and the Carba-NP method. Isolates were investigated for the most common carbapenemases encoding genes bla KPC , bla NDM , and bla OXA-48 using multiplex PCR. Molecular typing of carbapenem-resistant isolates was done by ERIC-PCR followed by sequencing of common resistance genes. The overall rate of CRBSI in our study was 3.6 per 1000 central venous catheter (CVC) days. Among 57 Gram-negative CRBSI isolates, Klebsiella pneumoniae (K. pneumoniae) was the most frequently isolated (27/57; 47.4%), of which more than 70% were resistant to Meropenem. Phenotypic tests for carbapenemases showed that 37.9% of isolates were positive by modified Hodge test and 63.8% by Carba-NP detection. Multiplex PCR assay detected the bla NDM in 28.6% of the isolates and bla KPC in 26.8%, bla NDM and bla KPC were detected together in the same isolate in 5.6%, while bla OXA-48 -like were not detected. ERIC-PCR detected limited genetic relatedness between K. pneumoniae isolates. Elevated resistance rates were observed to all antibiotics including carbapenems among K. pneumoniae isolates causing CRBSI. ERIC-PCR showed that the resistant isolates were mainly polyclonal. Our results call for reinforcement of antimicrobial stewardship and measures to prevent CRBSI. � 2018, Springer-Verlag GmbH Germany, part of Springer Nature.