Browsing by Author "El Bishbishy, Mahitab H"

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Chondroprotection of fruit peels in a monosodium iodoacetate‐induced osteoarthritis rat model via downregulation of Col1A1
(Wiely, 04/03/2022) Aborehab, Nora M; El Bishbishy, Mahitab H
The potential of the fruit peels of mango, orange, cantaloupe, and pomegranate in the treatment of osteoarthritis (OA) was evaluated in a rat model. Their metabolic profiles were characterized using ultrahigh‐performance liquid chromatography (UPLC)–electrospray ionization‐mass spectroscopy and 66 albino rats were intra‐ articularly injected with monosodium iodoacetate in the knee joints. The extracts were orally administered at doses of 200 and 400 mg/kg for 28 days. Serum levels of IL‐6 and tissue levels of cyclooxygenase‐2 (COX‐2), peroxisome proliferator‐ activated receptor‐gamma (PPARγ), and alpha‐smooth muscle actin (α‐SMA) were measured using ELISA. COL1A1 expression was measured by quantitative polymerase chain reaction. Histopathological changes in the joints were examined. In the extracts, 85 metabolites were annotated, and the levels of interleukin (IL)‐6, COX‐2, α‐SMA, malondialdehyde, and nitric oxide were significantly reduced, while PPARγ and glutathione levels were significantly raised in all treated groups compared to the OA group. All extracts downregulated the cartilage mRNA expressions for COL1A1 dose‐dependently. Mango peel extract exhibited the best chondroprotective effect. The in silico study showed the link between mango extract metabolites and COX‐2.
Downregulation of MMP1 expression mediates the anti-aging activity of Citrus sinensis peel extract nanoformulation in UV induced photoaging
(Elsevier, 2021-03) Amer, Reham I; Ezzat, Shahira M; Aborehab, Nora M; Ragab, Mai F; Mohamed, Dalia; Hashad, Amira; Attia, Dalia; Salama, Maha M; El Bishbishy, Mahitab H
Aging of the skin is a complicated bioprocess that is affected by constant exposure to ultraviolet irradiation. The application of herbal-based anti-aging creams is still the best choice for treatment. In the present study, Citrus sinensis L. fruit peels ethanolic extract (CSPE) was formulated into lipid nanoparticles (LNPs) anti-aging cream. Eight different formulations of CSEP-LNPs were prepared and optimized using 23 full factorial designs. In vivo antiaging effect of the best formula was tested in Swiss albino mice where photo-aging was induced by exposure to UV radiation. HPLC-QToF-MS/MS metabolic profiling of CSPE led to the identification of twenty-nine me- tabolites. CSPE was standardized to a hesperidin content of 15.53 ± 0.152 mg% using RP-HPLC. It was suggested that the optimized formulation (F7) had (245 nm) particle size, (91.065%) EE, and (91.385%) occlusive effect with a spherical and smooth surface. The visible appearance of UV-induced photoaging in mice was significantly improved after topical application on CSPE-NLC cream for 5 weeks, levels of collagen and SOD were significantly increased in CSPE- NLC group, while levels of PGE2, COX2, JNK, MDA, and elastin was reduced. Finally, The prepared anti-aging CSPE-NLC cream represents a safe, convenient, and promising skincare cosmetic product.
Metabolomics‑based profling of 4 avocado varieties using HPLC–MS/ MS andGC/MS and evaluation of their antidiabetic activity
(Springer nature, 23/03/2022) Younis, InasY; Khattab, Amira R; Selim, Nabil M; Sobeh, Mansour; Elhawary, Seham S; El Bishbishy, Mahitab H
Seven avocado “Persea americana” seeds belonging to 4 varieties, collected from diferent localities across the world, were profled using HPLC–MS/MS and GC/MS to explore the metabolic makeup variabilities and antidiabetic potential. For the frst time, 51 metabolites were tentatively- identifed via HPLC–MS/MS, belonging to diferent classes including favonoids, bifavonoids, naphthodianthrones, dihydrochalcones, phloroglucinols and phenolic acids while 68 un-saponifed and 26 saponifed compounds were identifed by GC/MS analysis. The primary metabolic variabilities existing among the diferent varieties were revealed via GC/MS-based metabolomics assisted by unsupervised pattern recognition methods. Fatty acid accumulations were proved as competent, and varietal-discriminatory metabolites. The antidiabetic potential of the diferent samples was explored using in-vitro amylase and glucosidase inhibition assays, which pointed out toGwen (KG) as the most potent antidiabetic sample. This could be attributed to its enriched content of poly-unsaturated fatty acids and polyphenolics. Molecular docking was then performed to predict the most promising phytoligands in KG variety to be posed as antidiabetic drug leads. The highest in-silico α-amylase inhibition was observed with chrysoeriol-4′-O-pentoside-7-O-rutinoside, apigenin-7-glucuronide and neoeriocitrin which might serve as potential drug leads for the discovery of new antidiabetic remedies.
Molecular Networking Leveraging the Secondary Metabolomes Space of Halophila stipulaceae (Forsk.) Aschers. and Thalassia hemprichii (Ehrenb. ex Solms) Asch. in Tandem with Their Chemosystematics and Antidiabetic Potentials
(MDPI, 2021-05) Hegazi, Nesrine M; Saad, Hamada H; Marzouk, Mona; Abdel Rahman, Mohamed F; El Bishbishy, Mahitab H; Zayed, Ahmed; Ulber, Roland; Ezzat, Shahira M
The Red Sea is one of the most biodiverse aquatic ecosystems. Notably, seagrasses possess a crucial ecological significance. Among them are the two taxa Halophila stipulacea (Forsk.) Aschers., and Thalassia hemprichii (Ehrenb. ex Solms) Asch., which were formally ranked together with the genus Enhalus in three separate families. Nevertheless, they have been recently classified as three subfamilies within Hydrocharitaceae. The interest of this study is to explore their metabolic profiles through ultra-high-performance liquid chromatography-high-resolution mass spectrometry (UPLC- HRMS/MS) analysis in synergism with molecular networking and to assess their chemosystematics relationship. A total of 144 metabolites were annotated, encompassing phenolic acids, flavonoids, terpenoids, and lipids. Furthermore, three new phenolic acids; methoxy benzoic acid-O-sulphate (16), O-caffeoyl-O-hydroxyl dimethoxy benzoyl tartaric acid (26), dimethoxy benzoic acid-O-sulphate (30), a new flavanone glycoside; hexahydroxy-monomethoxy flavanone-O-glucoside (28), and a new steviol glycoside; rebaudioside-O-acetate (96) were tentatively described. Additionally, the evaluation of the antidiabetic potential of both taxa displayed an inherited higher activity of H. stipulaceae in alleviating the oxidative stress and dyslipidemia associated with diabetes. Hence, the current research significantly suggested Halophila, Thalassia, and Enhalus categorization in three different taxonomic ranks based on their intergeneric and interspecific relationship among them and supported the consideration of seagrasses in natural antidiabetic studies.
Natural Products for the Management of Cardiovascular Diseases
(Bentham Science Publishers, 2020) Salem, Mohamed A; El Bishbishy, Mahitab H; Zayed, Ahmed; Mahrous, Amr A; Salama, Maha M; Ezzat, Shahira M
Cardiovascular diseases constitute a serious public health problem. It is estimated that they are responsible for nearly 30% of world mortality. Regardless of the developments in the diagnosis and management of cardiovascular diseases, their incidence rate remains increasing. Therefore, newlines of drugs are needed to manage the expanding population of patients with cardiovascular diseases. Even though the most common existing treatments for cardiovascular diseases are synthetic molecules, natural compounds, of different chemical classes, are also being tested. Medicinal plants have been employed in the treatment of some cardiovascular diseases such as congestive heart failure and hypertension many centuries ago. Recently, the traditional remedies application for the treatment of different disorders is gaining revived popularity. In this chapter, we will investigate the efficacy and safety of natural products under preclinical studies and clinical trials with particular emphasis on their traditional uses and implementations in the primary health care system. The great potential of medicinal plants and herbs will be discussed in light of the rising prevalence of cardiovascular diseases as one of the most devastating global health problems.
Untargeted metabolomics-based molecular networking for chemical characterization of selected Apiaceae fruit extracts in relation to their antioxidant and anti-cellulite potentials
(Elsevier, 2023-12) Hegazia, Nesrine M; Salemb, Mohamed A; Saad, Hamada H; Aborehab, Nora M; El Bishbishy, Mahitab H; Ezzat, Shahira M
Stress, obesity, hormonal changes, and aging have been connected to cellulite aggravation resulting in skin dimpled appearance, a very common painless skin disorder with a female preponderance. Several Apiaceae plants have been traditionally used for cosmetic applications. However, their screening for anti-cellulite potential has not been deeply investigated. In this work, UPLC-HRMS/MS coupled with molecular networking was employed to glean a holistic overview of the chemodiversity of the metabolome of nine Apiaceae fruits. Additionally, the extracts were screened for in vitro antioxidant and anti-cellulite activities. Apium graveolens and Petroselinum crispum revealed excellent free radical scavenging activity, remarkably increased lipolysis, and decreased adipogenesis. Furthermore, apigenin and its glycosides were identified to be the major components in both extracts, which might be responsible for the antioxidant activity and anti-cellulite potential. Conclusively, these results signify the potent antioxidant and anti-cellulite properties of A. graveolens and P. crispum fruit extracts, holding potential for the development of plant derived products for cellulite management.