Browsing by Author "Eid, Omneya"

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Comprehensive overview: the effect of using different solvents for barley extraction with its anti-inflammatory and anti-oxidant activity
(Wiley-Blackwell, 2023-02) Eid, Omneya; Elkady, Wafaa M.; Ezzat, Shahira M; El Sayed, Abeer; Abdelsattar, Essam
Barley (Hordeum vulgare L.) is one of the world's oldest cereal crops. There is considerable interest in barley's potential usage in human diets. Barley is rich in bioactive metabolites such as high content of β-glucan, fiber, and vitamin E. It is also well known as a rich source of phytochemical derivatives, namely, phenolic acids, flavonols, chalcones, flavones, proanthocyanidins, and flavanones. Phenolic compounds are recognized as an excellent dietary material with antioxidant and anti-inflammatory activities. This review was written to give an overview on the main components that are separated from barley using different solvents. Even though there were numerous biological activities for barely, the antioxidant as well as the anti-inflammatory were the main focus in this review.
Crassulaceae (chemistry and pharmacology) - A review
(ELSEVIER SCIENCE BV, 2018-12) Choucry, Mouchira; Gonaid, Mariam; Ezzat, Shahira M; Eid, Omneya
Family Crassulaceae comprises approximately 1410 species which are commonly used for ornamental purposes. And nowadays showed many medicinal values. Recently, it has had been reported that several species of the family possess certain biological and pharmacological activities as: anti-oxidant, antihyperglycemic, antimicrobial, antiulcerogenic, cytotoxic/anti-cancer, anti-inflammatory, anti-nociceptive activity, hepatoprotective, analgesic, anti-arthritic, anti-malarial, antimutagenic, insecticidal, anti-thrombolytic, antihypertensive and myometrial activities. Earlier studies on different Crassulaceae plants have reported the isolation of flavonoids, sterols, ascorbic acid, trace elements, organic acids, hydrocarbons, triterpenoids, phenolic components and bufadienolides. This review contains an overview on the chemistry and pharmacological activities of Family Crassulaceae. (c) 2018 Future University. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
Evaluation of antioxidant activity in diferent Egyptian barley cultivars: an in vitro and in silico study
(Springer open, 2024-05) Eid, Omneya; Ezzat, Shahira; Elkady, Wafaa M; El Sayed, Abeer; Abd el‑sattar, Essam
Background Cereals have historically played a crucial role in the human diet, serving as a signifcant natural source of energy and ofering various health benefts. Barley (Hordeum vulgare L.) has been given signifcant attention in recent years due to its exceptional nutritional value, surpassing that of other cereals. The objective of this research is to evaluate the antioxidant activity of various solvent extracts obtained from three diferent barley cultivars. Results The G.136 variety’s acetone extract exhibited the highest level of antioxidant activity in both the DPPH assay, with an IC50 of 55.62 µg/ml, and the FRAP assay, with 447 μM trolox/mg extract. The dominant compounds identi‑ fed before in the acetone fraction were subjected to an evaluation of their docking scores, along with an assessment of ADMET (Absorption, Distribution, Metabolism, Excretion, and Toxicity) and TOPKAT (Toxicity Prediction by Komputer Assisted Technology) studies. Notably, hordatine A1, prodelphinidin B3, hordatine B1, procyanidin B2, and isovitexin 7-O-glucoside were the major compounds with the highest LipDock scores compared to trolox the reference drug with polyphenol oxidase. Conclusions The fndings indicate that the acetone extract from all three cultivars demonstrates noteworthy results, surpassing the efcacy of other solvent extracts against the antioxidant activity.
Metabolomic profiling of barley extracts obtained via different solvents and evaluation of their anti-inflammatory efficacy
(Elsevier B.V, 2024-07) Eid, Omneya; Salem, Mohamed A; Mohamed, Osama G; Ezzat, Shahira; Tripathi, Ashootosh; Ewida, Menna A; El Sayed, Abeer; Abdel-Sattar, Essam; Elkady, Wafaa M
Barley (Hordeum vulgare L.) stands out for its special nutritional value compared to other cereals, garnering significant attention in recent years. In this study, a comprehensive chemical and biological evaluation of different barley cultivars extracted with different solvents was performed. Ultra-high performance liquid chromatography coupled with electrospray ionization quadrupole time-of-flight tandem mass spectrometry (UHPLC-ESI-QTOF-MS/MS) was used in the phytochemical study. The molecular docking with absorption, distribution, metabolism, excretion, and toxicity (ADMET) study and multivariate analysis alongside the quantification of total phenolic contents (TPC) were performed. Furthermore, the anti-inflammatory action was in vitro assessed by measuring COX-1, COX-2, and 5-LOX inhibition. This study seeks to establish a suitable solvent for extracting anti-inflammatory properties from diverse barley cultivars. The methanol extract obtained from the G.131 cultivar, followed by the 80% methanol extract from the G.136 cultivar, exhibited the most favorable IC50 values in comparison to the reference drugs indomethacin and celecoxib. The phytochemical profile revealed more than 60 compounds that belonged to nitrogen-containing compounds, phenolic compounds, organic acids, flavonoids, and their glycosides, hordatines, and other classes. The TPC and COX-2 inhibition was positively correlated with isoscoparin, coumaroyle–OH–eagmatine, isoorientin, isovitexin 7-O-rhamnosylglucoside, isoscoparin 7-O-glucoside, and isoorientin-7-O-[6-feruloyl]-glucoside. These findings along with their pharmacodynamics study suggested a potential synergistic and significant contribution of these compounds with the anti-inflammatory activity.
Novel Cycloartan derivative with genetic and metabolic proৎiling oftwo Crassulaceae species
(JK Welfare & Pharmascope Foundation, 2020-12) Eid, Omneya; Ezzat, Shahira M; Gonaid, Mariam; Choucry, Mouchira
and C. ovata respectively. The unsaponifiable matter (USM) of n-hexane extract of C. tetragona and C. ovata revealed the presence of 32 hydrocarbons with the presence of n-tricontane as the major hydrocarbon in both species, in addition to seven steroidal components in both species. The investigation of fatty acid methyl ester (FAME) revealed the presence of 12 components in C. tetragona and 9 components in C. ovata, and a novel triterpene, namely, 28 Methyl-5α-cycloart12, 20, 24-trien-15β-Ol was isolated and identified from MeCl together with 5 known compounds.