Browsing by Author "Ei-Bagary R."

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    Online pre-column derivatization with chromatographic separation to determine folic acid
    (2013) Emara S.; Masujima T.; Zarad W.; Kamal M.; Ei-Bagary R.; Pharmaceutical Chemistry Department; Faculty of Pharmacy; Misr International University; Km 28 Ismailia Road; Cairo; Egypt; Analytical Molecular Medicine and Devices Laboratory; Hiroshima University; Graduate School of Biomedical Sciences; 1-2-3; Kasumi; Minami-ku; Hiroshima; 734-8551; Japan; Pharmaceutical Analytical Chemistry Department; Faculty of Pharmacy; Modern Sciences and Arts University; 26 July Mehwar Road intersection with Wahat Road; 6 October City; Egypt; Pharmaceutical Chemistry Department; Faculty of Pharmacy; Cairo University; Kasr El Aini St.; Cairo 11562; Egypt
    A simple, sensitive, and selective online pre-column derivatization high-performance liquid chromatographic method was developed and validated for the first time to determine trace levels of folic acid (FA). An oxidant cerium (IV) trihydroxyhydroperoxide packed reactor was used for pre-column oxidation and was combined by column switching with a C18 analytical column for sample enrichment and separation. The method was based on oxidative cleavage of FA into highly fluorescence products, 2-amino-4-hydroxypteridine-6-carboxaldehyde and the corresponding 2-amino-4-hydroxypteridine-6-carboxylic acid, during the flow of 0.04 M phosphate buffer (pH 3.5) containing the analyte through packed reactor at a flow rate of 0.2 mL/min and 40�C. The fluorescent products were enriched on the head of the analytical column for the final separation. The separation was performed at room temperature using a mobile phase consisting of phosphate buffer (0.04 M, pH 3.5) and acetonitrile (90:10, v/v). The eluents were monitored at emission and excitation wavelengths of 463 and 367 nm, respectively. The method showed excellent recovery, precision and accuracy with detection limits of 0.067 ng/mL from 500 L of sample FA. The developed method was successfully applied to the determination of FA in pharmaceutical formulations and showed a recovery of 99.31% and a relative standard deviation of 1.72%. 2012 � The Author [2012].