Browsing by Author "Diab, AA"

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    Design, Synthesis, Characterization and Toxicity Studies of Poly (N-IsoPropylacrylamide-co-Lucifer Yellow) Particles for Drug Delivery
    (2016) Snowden, MJ; Diab, AA; Mitchell, JC; Richardson, SCW; Alexander, BD; Mohsen, R
    A novel fluorescent temperature/pH responsive particle was designed, synthesized, characterised and tested for toxicity. Poly (N-iso-propylacrylamide-co-5%-lucifer yellow) (p(NIPAM-co-5% LY)) was prepared using a surfactant free emulsion polymerisation technique. The particles were negatively charged and were approximately 250 nm at 15°C. When the particles de-swell following an increase in temperature, a particle size around 100 nm is obtained. The toxicity of different concentrations of the new particles (p(NIPAM)-co-5% LY, as well as the 100% p(NIPAM) and the main monomer NIPAM was tested on two cell lines (Hela and Vero). The toxicity was tested in comparison to a positive control (dextran sugar) and a negative one (poly(ethylenimine)) (PEI). The results show that the two particles show cell viability over 80% (for both cell lines Hela and Vero) up to a concentration of 3 mg/mL while NIPAM monomer showed cell viability over 80% at a concentration equal to or less than 0.3 mg/mL. The fluorescence property of the novel particles make them traceable. Combining this property (tracing) to the ability of the particles to release their content in response to temperature and pH change can be a potential drug delivery system for cancer treatment.
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    Development of transgenic Egyptian cotton (Gossypium barbadense) varieties from meristematic tissue.
    (National Cotton Council, 2000) Momtaz, OA; Diab, AA; Madkour, MA
    A variety-independent protocol was developed for genetic transformation and regeneration of Egyptian cotton (Gossypium barbadense) plants from meristematic tissue of excised embryonic axes and from the shoot apical meristem of seedlings using the Bio-Rad PDS/1000/He gene gun. Tungsten beads coated with plasmid DNA (pBI221) carrying the coding sequence for the GUS protein were used to deliver the gene directly into the meristematic tissue. Transgenic cotton plants were obtained using techniques of shoot meristem transformation and direct regeneration of shoots and roots. Plants derived from this process were screened for integration of the GUS gene into the plant genome using dot blotting, genomic Southern analysis, immunoblot hybridization and histochemical assay. This work represents the first report of shoot meristem genetic transformation and regeneration of commercial varieties (Giza 45, Giza 87 and Giza 88 as extra-long staple varieties and Giza 85, Giza 89 and Giza 86 as long staple varieties) using biolistic gun transformation. Molecular and genetic characterization of primary transformants and their progeny proved that GUS gene was successfully integrated into the genome.
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    Genetic diversity of some Egyptian durum wheat cultivars
    (MARSLAND PRESS, 2011) Fouda, AH; Gad, KIM; Diab, AA; Safwat, G; Hussein, MH
    The objective of this investigation was to assess the genetic diversity among three Egyptian durum wheat i.e. Beni Suif 4, Beni Suif 5 and Beni Suif 6 and one bread wheat i.e. Sids 12 cultivars using sodium dodecyle sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and randomly amplified polymorphic (RAPD) markers. Protein electrophoreses showed that Beni Suif 4 was characterized by the absence of band-3 with 41.56 kDa. RAPD analysis showed that the number of polymorphic amplicons was 56 out of a total of 93 amplicons, thus revealing a level of 60.0% polymorphism. The highest genetic similarity revealed by RAPD analysis (95.0%) was between Beni Suif 5 and Beni suif 6. While, lowest similarity (65%) was found between Beni Suif 4 and Beni Suif 5. The dendrogram separated Sids 12 from all the other genotypes, thus demonstrating the distinctiveness of the genetic background of this genotype from all the other genotypes. The three genotypes constituted a subcluster divided into two groups, one group composed of Beni Suif 5 and Beni Suif 6, while the second group comprised Beni Suif 4.
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    Production, Characterization and Application of a New Biosurfactant Derived from Egyptian Sunflower Seeds
    (Int. J. of Agrci. Sci. and Res, 2016) Diab, A; Sami, Shereen; Diab, AA
    A new biosurfactant (SR122) was obtained for the first time from the seeds of sunflower plant growing locally in Egypt. This bioproduct was recovered from the seeds using simple and cost effective method that gave higher biosurfactant yield (20-25% w/w). The new product (SR 122) may be an alternative to the microbial and synthetic surfactants because it is non toxic (as it was originated from safe natural sources- the plant), cost-effective, produced in higher yield (as compared to the microbial biosurfactant) and may acquire public acceptance. The high surface activity of SR 122 (as measured by the ODA method, surface tension reduction and E24 method), the non-toxicity and tolerance to wide range of harsh condition of temperatures (up to 121ºc), pH values (2-11) and different NaCl concentrations (2% -25% w/v) make this new product a promising candidate for the application in many industries and for the protection of the environment. On a large laboratory scale SR122 was applied for cleaning oil storage tank. During the cleaning process more than 95% of the oily sludge was recovered and the viscosity of the recovered oil was decreased allowing the oily sludge to be easily floated on the top of the biosurfactant as a distinct phase. This product SR 122 was offered for a patent no: 1289/2014