Browsing by Author "Dawoud M.H.S."

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Comparative lyophilized platelet-rich plasma wafer and powder for wound-healing enhancement: formulation, in vitro and in vivo studies
(Taylor and Francis Ltd., 2019) Yassin G.E.; Dawoud M.H.S.; Wasfi R.; Maher A.; Fayez A.M.; Department of Pharmaceutics and Industrial Pharmacy; Faculty of Pharmacy; Al Azhar University; Cairo; Egypt; Department of Pharmaceutics; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA University); Giza; Egypt; Department of Microbiology; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA University); Giza; Egypt; Department of Biochemistry; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA University); Giza; Egypt; Department of Pharmacology; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA University); Giza; Egypt
Platelet-rich plasma (PRP) accelerates wound healing, as it is an excellent source of growth factors. PRP was separated from whole human blood by centrifugation. PRP powder and wafers were prepared by lyophilization, with the wafers prepared using sodium carboxymethylcellulose (Na CMC). The PRP wafers showed porous structures, as indicated by scanning electron microscopy (SEM) images, and the ability of the wafer to absorb exudates and thus promote wound healing was tested with the hydration capacity test. The platelet count was tested and indicated that the presence of PRP in the wafers had no effect on the platelet count. An antimicrobial activity test was carried out, showing that PRP had antibacterial activity against Gram-negative bacteria. Compared with lyophilized PRP powder and PRP-free wafers, PRP wafers showed the highest percent of wound size reduction on induced wounds in rats. Histopathological examination of rat skin showed that the PRP wafers achieved the shortest healing time, followed by the lyophilized PRP powder and finally the PRP-free wafers. The present study revealed that PRP can be formulated as a wafer, which is a promising pharmaceutical delivery system that can be used for enhanced wound-healing activity and improved the ease of application compared to lyophilized PRP powder. 2019, 2019 Informa UK Limited, trading as Taylor & Francis Group.
Enhancement of the bioavailability of an antihypertensive drug by transdermal protransfersomal system: formulation and in vivo study
(Taylor and Francis Ltd, 2018) Morsi N.M.; Aboelwafa A.A.; Dawoud M.H.S.; Department of Pharmaceutics and Industrial Pharmacy; Faculty of Pharmacy; Cairo University; Cairo; Egypt; Department of Pharmaceutics; Faculty of Pharmacy; October University for Modern Sciences and Arts; Dokki; Egypt
Timolol Maleate (TiM), a nonselective ?-adrenergic blocker, is a potent highly effective agent for management of hypertension. The drug suffers from poor oral bioavailability (50%) due to its first pass effect and a short elimination half-life of 4 h; resulting in its frequent administration. Transdermal formulation may circumvent these problems in the form of protransfersomes. The aim of this study is to develop and optimize transdermal protransfersomal system of Timolol Maleate by film deposition on carrier method where protransfersomes were converted to transfersomes upon skin hydration following transdermal application under occlusive conditions. Two 2 3 full factorial designs were employed to investigate the influence of three formulation variables which were; phosphatidyl choline: surfactant molar ratio, carrier: mixture and the type of SAA each on particle size, drug entrapment efficiency and release rate. The optimized formulation was evaluated regarding permeation through hairless rat skin and compared with oral administration of aqueous solution on male Wistar rats. Optimized protransfersomal system had excellent permeation rate through shaved rat skin (780.69 ?g/cm 2 /h) and showed six times increase in relative bioavailability with prolonged plasma profile up to 72 h. A potential protransfresomal transdermal system was successfully developed and factorial design was found to be a smart tool in its optimization. � 2017 Informa UK Limited, trading as Taylor & Francis Group.
Improved bioavailability of timolol maleate via transdermal transfersomal gel: Statistical optimization, characterization, and pharmacokinetic assessment
(Elsevier, 2016) Morsi N.M.; Aboelwafa A.A.; Dawoud M.H.S.; Department of Pharmaceutics and Industrial Pharmacy; Faculty of Pharmacy; Cairo University; Cairo; Egypt; Department of Pharmaceutics; Faculty of Pharmacy; Modern Sciences and Arts University; Cairo; Egypt
Timolol maleate (TiM), a nonselective ?-adrenergic blocker, is a potent highly effective agent for management of hypertension. The drug suffers from extensive first pass effect, resulting in a reduction of oral bioavailability (F%) to 50% and a short elimination half-life of 4 h; parameters necessitating its frequent administration. The current study was therefore, designed to formulate and optimize the transfersomal TiM gel for transdermal delivery. TiM loaded transfersomal gel was optimized using two 23 full factorial designs; where the effects of egg phosphatidyl choline (PC): surfactant (SAA) molar ratio, solvent volumetric ratio, and the drug amount were evaluated. The formulation variables; including particle size, drug entrapment efficiency (%EE), and release rate were characterized. The optimized transfersomal gel was prepared with 4.65:1 PC:SAA molar ratio, 3:1 solvent volumetric ratio, and 13 mg drug amount with particle size of 2.722 ?m, %EE of 39.96%, and a release rate of 134.49 ?g/cm2/h. The permeation rate of the optimized formulation through the rat skin was excellent (151.53 ?g/cm2/h) and showed four times increase in relative bioavailability with prolonged plasma profile up to 72 h compared with oral aqueous solution. In conclusion, a potential transfersomal transdermal system was successfully developed and the factorial design was found to be a smart tool, when optimized. � 2016
Insulin Mucoadhesive Liposomal Gel for Wound Healing: a Formulation with Sustained Release and Extended Stability Using Quality by Design Approach
(Springer New York LLC, 2019) Dawoud M.H.S.; Yassin G.E.; Ghorab D.M.; Morsi N.M.; Department of Pharmaceutics; Faculty of Pharmacy; October University for Modern Sciences and Arts; (MSA University); Giza; Egypt; Department of Pharmaceutics and Industrial Pharmacy; Faculty of Pharmacy; Al-Azhar University; Cairo; Egypt; Department of Pharmaceutics and Industrial Pharmacy; Faculty of Pharmacy; Cairo University; Cairo; Egypt
The present study deals with the formulation of topical insulin for wound healing with extended stability and sustained release, by applying quality by design concepts. Insulin has been promoted as a promising therapeutic wound healing agent. Topical formulation of insulin faced major problems, as it cannot be delivered safely to the wound with a controlled rate. Formulation of insulin-loaded vesicles in optimized bio-adhesive hydrogels has been explored to ensure a safe delivery of insulin to wounds in a controlled manner. Quality by design (QbD) was applied to study the effect of several critical process parameters on the critical quality attributes. Ishikawa diagram was used to identify the highest risk factors, which were screened by a fractional factorial design and augmented by Box�Behnken design. The optimized formula was incorporated into a mucoadhesive gel, which was further subjected to stability and clinical studies. An optimized formula was obtained with a particle size of 257.751�nm, zeta potential ? 20.548�mv, 87.379% entrapment efficiency, and a release rate of 91.521�?g/cm2/h. The results showed that liposomal insulin remained stable for 6�months in aqueous dispersion state at 4�C. Moreover, the release was sustained up to 24�h. The clinical study showed an improvement in the wound healing rate, 16 times, as the control group, with magnificent reduction in the erythema of the ulcer and no signs of hypoglycemia. Insulin-loaded liposomal chitosan gel showed a promising drug delivery system with high stability and sustained release. � 2019, American Association of Pharmaceutical Scientists.
Response surface optimization and in-vitro evaluation of sustained release topical insulin liposomal spray for wound healing
(Open Science Publishers LLP Inc., 2018) Dawoud M.H.S.; Yassin G.E.; Ghorab D.M.; Morsi N.M.; Department of Pharmaceutics; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA University); Cairo; Egypt; Department of Pharmaceutics and Industrial Pharmacy; Faculty of Pharmacy; Al-Azhar University; Cairo; Egypt; Department of Pharmaceutics and Industrial Pharmacy; Faculty of Pharmacy; Cairo University; Cairo; Egypt
Chronic wounds are considered a major health care concern, that represents a life-threatening problem worldwide. Insulin has proven its great efficiency as a wound healing agent, especially with diabetic ulcers. However; insulin suffers degradation at the application site due to proteases, moreover; when wounds are painful the patient fails to apply any remedy frequently. In the present study, insulin has been formulated as a spray in liposomes, which protects it at the wound area and sustains its release and thus reducing the application frequency, furthermore; the spray reducing the direct contact of the applicator with the skin, thus, reducing the probability of infection. The full-factorial design has been applied in the preparation optimization of liposomes, where the effects of the cholesterol, method of preparation and sonication have been tested on the particle size and the entrapment efficiency. The present study shows how the thin film hydration method in the absence of cholesterol and sonication were the best conditions for insulin. liposomal formulation, that satisfies the target of the study. Liposomes showed a sustained release of insulin up to 24 hours and were successfully formulated into a spray dosage form. In conclusion, topical insulin liposomal spray offers a protective method from insulin degradation with an expected increase in the patient compliance. � 2018 Marwa H. S. Dawoud et al.
Topical insulin-liposomal formulation in management of recurrent aphthous ulcers: A randomized placebo-controlled trial
(NLM (Medline), 2019) El-Wakeel N.M.; Dawoud M.H.S.; Department of Oral Medicine and Periodontology; Faculty of Dentistry; October University for Modern Sciences and Arts (MSA)Cairo; Egypt; Faculty of Dentistry; Al-Azhar University (Girls Branch)Cairo; Egypt; Department of Pharmaceutics; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA )Cairo; Egypt
AIM: To evaluate the effectiveness of topical insulin-liposomal gel in aphthous ulcer treatment. METHODS: 80 participants with minor aphthous ulcers were randomly divided to receive either topical insulin-liposomal gel or placebo gel (once daily) for 6�days. Assessment of outcomes included visual analog scale (VAS) for pain (primary outcome), and secondary outcomes included ulcer duration and impact of treatment on quality of life using the Oral Health Impact Profile 14 (OHIP-14). Testing of the outcomes was carried out at 1, 2, 3, 4 and 6�days after treatment for VAS and at 6�days for OHIP-14. RESULTS: For pain scores, the test group showed a significant decrease by time, this was evident from day 1 (P�<�.001); at day 3, median and interquartile range (IQR) values were 0 (0-1). For the placebo group, a non-significant change by time was reported between baseline and day 1; at day 3, the median value was 7 (IQR, 7-9). The test group showed significantly lower mean duration than the placebo group (P�<�.001). OHIP-14 scores after 6�days showed that the test group had a significantly lower score than placebo (P�<�.001). CONCLUSIONS: Topical insulin-liposomal formulation showed marked effectiveness in management of aphthous ulcers. � 2019 John Wiley & Sons Australia, Ltd.