Browsing by Author "D. El-Gindi, Omayma"

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    Production of Flavonoids and Phenolic Compounds by Elicitation of Iphiona mucronata (Forssk.) Asch. & Schweinf (Asteraceae) Callus and Suspension Cultures
    (International Journal of Pharmacognosy and Phytochemistry, 2015) A. Al-Gendy, Amal; O. Bakr, Riham; D. El-Gindi, Omayma
    Callus and suspension cultures of Iphiona mucronata grown on Murashige and Skoog medium, supplemented with 0.1 mg/l kinetin (Kn), 0.1 mg/l naphthalene acetic acid (NAA) and 5 mg/l ascorbic acid were elicited with different elicitors. Total flavonoids and phenolics were determined by aluminum chloride - potassium acetate and Folin-Ciocalteu colorimetric methods respectively. When callus and suspension cultures elicited with 10 mg/l yeast extract (Y), it showed an increase of 2.8 and 3.7 folds for flavonoids production respectively. When callus cultures treated with 0.001 mM phenyl alanine (Ph), a slight increase in flavonoids content reached 1.17 folds, while treated suspension reached maximum level after 60 hours. Methyl jasmonate (MJ; 150 µM) increased flavonoids concentration 4 times for callus and 2.3 times for suspension. Determination of phenolics for elicited suspension showed an increase of 2.3 (Y), 2.5 (Ph) and 1.3 (MJ) times. The obtained results favored the use of yeast extract for high productivity and viability of cell lines.