Browsing by Author "Barbour, Elie K"

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    Strigol1/albumin/chitosan nanoparticles decrease cell viability, induce apoptosis and alter metabolomics profile in HepG2 cancer cell line
    (Elsevier Masson s.r.l., 2024-06) Al-Malki, Abdulrahman L; Bakkar, Ashraf; Huwait, Etimad A; Barbour, Elie K; Abulnaja, Kalid O; Kumosani, Taha A; Moselhy, Said S
    This article has been retracted: please see Elsevier Policy on Article Withdrawal (https://www.elsevier.com/locate/withdrawalpolicy). This article has been retracted at the request of the Editor-in-Chief. The journal was alerted about issues with images within Figure 3a, which appear to have multiple duplications, as detailed here: PubPeer - Strigol1/albumin/chitosan nanoparticles decrease cell viabil…. The journal requested the authors to provide an explanation to these concerns and the associated raw data, but this request was not satisfactorily fulfilled. The Editor-in-Chief assessed the case and decided to retract the article.
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    Strigol1/albumin/chitosan nanoparticles decrease cell viability, induce apoptosis and alter metabolomics profile in HepG2 cancer cell line
    (Elsevier, 08/02/2021) Al-Malki, Abdulrahman L; Bakkar, Ashraf; Huwait, Etimad; Barbour, Elie K; Abulnaja, Kalid O; Kumosani, Taha A; Moselhy, Said S
    Biomedicine & Pharmacotherapy Volume 142, October 2021, 111960 Strigol1/albumin/chitosan nanoparticles decrease cell viability, induce apoptosis and alter metabolomics profile in HepG2 cancer cell line Author links open overlay panelAbdulrahman L.Al-MalkiabcAshrafBakkardEtimad A.HuwaitaElie K.BarbourabeKalid O.AbulnajaabcTaha A.KumosaniabfSaid S.Moselhyg a Biochemistry Department, Faculty of Science, King Abdulaziz University (KAU), Jeddah, Saudi Arabia b Experimental Biochemistry Unit, King Fahd Medical Research Centre, King Abdulaziz University, Saudi Arabia c Bioactive Natural Products Research Group, King Abdulaziz University. Jeddah, Saudi Arabia d Modern Sciences and Arts University (MSA), 6th October, Giza, Egypt e Director of R and D Department, Opticon Hygiene Consulting, Oechsli 7, 8807 Freienbach, Switzerland f Production of Bio-products for Industrial Applications Research Group, King Abdulaziz University g Biochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt Received 16 March 2021, Revised 21 July 2021, Accepted 22 July 2021, Available online 2 August 2021. crossmark-logo https://doi.org/10.1016/j.biopha.2021.111960 Get rights and content Under a Creative Commons licenseopen access Highlights • Novel strigol nanoparticles (S/A/CNP) exhibit antiproliferative activity against HepG2 cells through dose dependent. • The S/A/CNP significantly reduces Krebs cycle intermediate and decrease glutamine level as energy source substrate. • The S/A/CNP induce morphological changes and trigger pro-apoptotic effects in HepG2. • The S/A/CNP reduces spermine and spermidine and inhibit DNA replication. Abstract Hepatocellular carcinoma is one of the most common causes of cancer-related deaths globally. Bioavailable, effective and safe therapeutic agents are urgently needed for cancer treatment. This study evaluated the metabolomics profiling, anti-proliferative and pro-apoptotic effects of strigol/albumin/chitosan nanoparticles (S/A/CNP) on HepG2 cell line. The diameter of S/A/CNP was (5 ± 0.01) nm. The IC50 was 180.4 nM and 47.6 nM for Strigol1 and S/A/CNP, respectively, after incubation for 24 h with HepG2 cells. By increasing the concentration of S/A/CNP, there was chromatin condensation, degranulation in the cytoplasm and shrinking in cell size indicating pro-apoptotic activity. Metabolomics profiling of the exposed cells by LC/MS/MS revealed that S/A/CNP up-regulated epigenetic intermediates (spermine and spermidine) and down-regulated energy production pathway and significantly decreased glutamine (P < 0.001). These findings demonstrated that S/A/CNP has anti-proliferative, apoptotic effects and modulate energetic, and epigenetic metabolites in the hepatocellular carcinoma cell line (HepG2).