Browsing by Author "Bakeer R.M."

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    Effects of sulforaphane on D-galactose-induced liver aging in rats: Role of keap-1/nrf-2 pathway.
    (Elsevier B.V., 2019) Saleh D.O.; Mansour D.F.; Hashad I.M.; Bakeer R.M.; Department of Pharmacology; Medical Research Division; The National Research Centre; 33 EL Bohouth St. (former EL Tahrir St.); P.O. 12622; Dokki; Giza; Egypt; Department of Clinical Pharmacy and Pharmacy Practice; Faculty of Pharmacy; Ahram Canadian University; Egypt; Clinical Biochemistry Unit; Faculty of Pharmacy and Biotechnology; German University in Cairo; Cairo; Egypt; Department of Pathology; Faculty of Medicine; Helwan University; Egypt; Instructor of Pathology; October University of Modern Sciences and Arts (MSA)University; Egypt
    Aging; a biological phenomenon characterized by progressive decline in cellular functions, is considered as a major risk factor of various liver diseases that plays as an adverse prognostic role, thus increasing mortality rate. However, diet is the main environmental factor that has a major impact on the aging process whereas; sulforaphane (SFN), an isothiocyanate organosulfur compound in cruciferous vegetables, has been reported with myriad biological effects. In the present study, SFN antiaging properties were evaluated on D-galactose (D-Gal)-induced liver aging in rats. For this purpose, forty adult male Wistar rats were divided into five groups. All animals, except the normal control, were intraperitoneally injected with D-Gal (300 mg/kg/day for 5 days a week)for six consecutive weeks. In the hepatoprotective groups, animals received oral SFN (0.5, 1.0 and 2.0 mg/kg)for 6 weeks concurrently with D-GAL. SFN administration improved liver biomarkers through decreasing serum levels of AST, ALT, total and direct bilirubin when compared to D-Gal-aging group. SFN significantly increased hepatic GSH level as well as catalase and glutathione-S-transferase activities while counteracted the elevation in hepatic oxidative stress markers; MDA, NO and protein carbonyl in aged rats. SFN abrogated the dysregulation in hepatic Keap-1, Nrf-2 and HO-1and limited the elevation of TNF-? and TGF-? concentrations in aging liver. Histopathologically, SFN decreased the intensity of hepatic fibrous proliferation in D-Gal-induced aging. In conclusion, SFN has shown hepatic anti-aging potential through promoting the antioxidant machinery via regulating Keap-1, Nrf-2 and HO-1 and antioxidant enzyme activities as well as ameliorating oxidative stress, hampering the inflammatory cytokines; TNF-? and TGF-?, and limiting hepatic fibrosis in a dose dependent manner. � 2019 Elsevier B.V.