Browsing by Author "Azmy, Ahmed Farag"

Filter results by typing the first few letters
Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Antimicrobial and Antibiofilm Activity of Monolaurin against Methicillin-Resistant Staphylococcus aureus Isolated from Wound Infections
    (Wiley, 2024-08) Abd El-Ghany, Shimaa Salah Hassan; Azmy, Ahmed Farag; EL-Gendy, Ahmed Osama; Abd El-Baky, Rehab Mahmoud; Mustafa, Ahmad; Abourehab, Mohammed A. S; El-Beeh, Mohamed E; Ibrahem, Reham Ali
    Background. Methicillin-resistant Staphylococcus aureus (MRSA) is one of the major pathogens associated with life-threatening infections, showing resistance to various antibiotics. Tis study aimed to assess the infuence of monolaurin on bioflm-forming MRSA. Methods. Te agar dilution method determined the minimum inhibitory concentration (MIC) of monolaurin against MRSA isolates and explored its impact on the resistance profle of selected antibiotics. Te assessment of combined therapy involving monolaurin and antibiotics was conducted using fractional inhibitory concentration (FIC). Te tissue culture plate strategy appraised monolaurin’s antibioflm activity and its inhibitory concentration (IC50), with assessment via scanning electron microscopy. Reverse transcription polymerase chain reaction (RT-PCR) discerned a monolaurin efect on the expression of the icaD gene. Results. Monolaurin exhibited MIC values ranging from 500 to 2000 μg/mL. FIC index showed a synergistic efect of monolaurin with β-lactam antibiotics ranging from 0.0039 to 0.25 (p < 0.001). Among the 103 investigated MRSA isolates, 44 (44.7%) displayed moderate bioflm formation, while 59 (55.3%) were strong bioflm producers. Antibioflm activity demonstrated concentration dependence, confrming monolaurin’s capacity to inhibit bioflm formation and exhibited strong eradicating efects against preformed MRSA bioflms with IC50 values of 203.6 μg/mL and 379.3 μg/mL, respectively. Scanning electron microscope analysis revealed reduced cell attachments and diminished bioflm formation compared to the control. Te expression levels of the icaD gene were remarkably reduced at monolaurin concentrations of 250 and 500 μg/mL. Conclusion. Monolaurin had signifcant inhibitory efects on MRSA pre-existing bioflms as well as bioflm development. So, it can be employed in the treatment of severe infections, particularly those associated with bioflm formation including catheter-associated infections.
  • Thumbnail Image
    Item
    Novel synergistic interactions between monolaurin, a mono-acyl glycerol and β lactam antibiotics against Staphylococcus aureus: an in vitro study
    (BioMed Central Ltd, 2024-12) Abd El Ghany, Shimaa Salah Hassan; Ibrahem, Reham A; EL‑Gendy, Ahmed Osama; Abd El‑Baky, Rehab Mahmoud; Mustafa, Ahmad; Azmy, Ahmed Farag
    Background A major worldwide health issue is the rising frequency of resistance of bacteria.Drug combinations are a winning strategy in fghting resistant bacteria and might help in protecting the existing drugs.Monolaurin is natural compound extracted from coconut oil and has a promising antimicrobial activity against Staphylococcus.aureus. This study aims to examine the efcacy of monolaurin both individually and in combination with β-lactam antibiotics against Staphylococcus aureus isolates. Methods Agar dilution method was used for determination of minimum inhibitory concentration (MIC) of monolau‑ rin against S.aureus isolates. Scanning electron microscope (SEM) was used to detect morphological changes in S. aureus after treatment with monolaurin. Conventional and Real-time Polymerase chain reaction (RT-PCR) were per‑ formed to detect of beta-lactamase (blaZ) gene and its expressional levels after monolaurin treatment. Combination therapy of monolaurin and antibiotics was assessed through fractional inhibitory concentration and time-kill method. Results The antibacterial activity of monolaurin was assessed on 115 S.aureus isolates, the MIC of monolaurin were 250 to 2000 µg/ml. SEM showed cell elongation and swelling in the outer membrane of S.aureus in the prescence of 1xMIC of monolaurin. blaZ gene was found in 73.9% of S.aureus isolates. RT-PCR shows a signifcant decrease in of blaZ gene expression at 250 and 500 µg/ml of monolaurin. Synergistic efects were detected through FIC method and time killing curve. Combination therapy established a signifcant reduction on the MIC value. The col‑ lective fndings from the antibiotic combinations with monolaurin indicated synergism rates ranging from 83.3% to 100%.In time-kill studies, combination of monolaurin and β-lactam antibiotics produced a synergistic efect. Conclusion This study showed that monolaurin may be a natural antibacterial agent against S. aureus, and may be an outstanding modulator of β-lactam drugs. The concurrent application of monolaurin and β-lactam antibiotics, exhibiting synergistic efects against S. aureus in vitro, holds promise as potential candidates for the development of combination therapies that target particularly, patients with bacterial infections that are nearly incurable.