Browsing by Author "Ayoub N.A."

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    Chemical Composition and Biological Activities of the Essential Oil from Leaves and Flowers of Pulicaria incisa sub. candolleana (Family Asteraceae)
    (Institute of Physics Publishing, 2017) Shahat E.A.; Bakr R.O.; Eldahshan O.A.; Ayoub N.A.; Pharmacognosy Department; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); Giza; Egypt; Pharmacognosy Department; Faculty of Pharmacy; Ain Shams University; Cairo; Egypt; Pharmacology Department; Faculty of Medicine; Umm Al-Qura University; Makkah; 24382; Saudi Arabia
    The composition of the essential oil isolated from leaves and flowers of Pulicaria incisa sub. candolleana E. Gamal-Eldin, growing in Egypt, was analysed by GC and GC-MS. Forty-nine and 68 compounds were identified from the oils of the leaves and flowers accounting for 86.69 and 84.29%, respectively of the total detected constituents. Both leaves and flowers oils were characterized by the high content of carvotanacetone with 66.01, 50.87 and chrysanthenone 13.26, 24.3%, respectively. The cytotoxic activity of both essential oils was evaluated against hepatocellular carcinoma cell line HEPG-2, using MTT assay and vinblastine as a reference drug. Leaf oil showed higher activity with IC50 11.4�?g/ml compared with 37.4�?g/ml for flower oil. The antimicrobial activity of both oils was evaluated using agar well diffusion method towards two representatives for each of Gram positive and Gram negative bacteria as well as four representatives for fungi. The minimum inhibitory concentration of both essential oils against bacterial and fungal strains was obtained in the range of 0.49���15.63�?g/ml. � 2017 Wiley-VHCA AG, Zurich, Switzerland
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    Glucosinolates profile, volatile constituents, antimicrobial, and cytotoxic activities of Lobularia libyca
    (Institute of Electrical and Electronics Engineers Inc., 2016) Al-Gendy A.A.; Nematallah K.A.; Zaghloul S.S.; Ayoub N.A.; Faculty of Pharmacy; October University for Modern Sciences and Arts (MSA); Giza; Egypt; Faculty of Pharmacy; Zagazig University; Zagazig; Egypt; Faculty of Pharmacy; British University in Egypt; Cairo; Egypt; Faculty of Pharmacy; Ain-Shams University; Cairo; Egypt; Faculty of Medicine; Umm Al-Qura University; Makkah; Saudi Arabia
    Context: Brassicaceae plants are associated with protection against cancers due to their glucosinolate contents. Objectives: We investigate fresh leaves, roots and ripe seeds of Lobularia libyca (Viv.) C.F.W. Meissn. (Brassicaceae) to identify their glucosinolate constituents, antimicrobial and cytotoxic activities Materials and methods: The glucosinolates were identified using GC-MS analysis of their hydrolysis products and LC-MS analysis in the case of seeds. Disc diffusion (1 mg/disc) and minimum inhibitory concentration (0�160 ?g/mL) methods were used to evaluate the antimicrobial activity of seed hydrolysate. In vitro cytotoxicity against colorectal HCT-116, hepatic HUH-7, breast MCF-7 and lung A-549 cells was evaluated for seed hydrolysate (0.01�100 ?g/mL) using the sulforhodamine B assay and doxorubicin as a standard Results: Three glucosinolates were identified for the first time in this plant and genus Lobularia. Glucoiberverin was the major compound accumulated in the seeds and leaves, while glucoiberin and glucoerucin were detected only in the seeds. No glucosinolates were detected in roots under the same experimental conditions. Other volatile constituents, e.g., terpenes and fatty acids were only identified in the seeds. The seed hydrolysate showed significant antimicrobial activities against Candida albicans and Pseudomonas aeruoginosa (MIC = 64 and 82 ?g/mL, respectively). The seed hydrolysate exhibited a marked selective cytotoxicity in vitro against colorectal, hepatic and breast cancer cell lines. The IC 50 values were 0.31, 2.25 and 37 ?g/mL, respectively. Discussion and conclusion: The results indicated the antimicrobial activity of L. libyca and the selective effect of the seed hydrolysate as a cytotoxic drug that is potentially more active than doxorubicin against HCT-116. � 2016 Informa UK Limited, trading as Taylor & Francis Group.