Browsing by Author "Alyaa Farid"

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    Evaluation of the effect of grape seed extract loaded-chitosan nanoparticles on cryptosporidiosis in dexamethasone immunosuppressed male mice
    (Elsevier B.V, 2025-02-25) Karim Tarek; Gehan Safwat; Alyaa Farid
    Cryptosporidiosis is a worldwide health problem that results in an economic loss. The disease is caused by the protozoan Cryptosporidium spp. Individuals with suppressed immunity, like those with organ transplantation, cancer and human immunodeficiency virus syndrome, suffer from the infection that may lead to the death. Nitazoxanide (NTZ) is the approved FDA treatment for cryptosporidiosis in immunocompetent individuals. There is an urgent need to find a new natural treatment that can replace NTZ in immunosuppressed hosts. The study aimed to use grape seed extract loaded chitosan nanoparticles (GSEx-CHNPs) in treatment of cryptosporidiosis in immunosuppressed male mice. GSEx was prepared by the alcoholic extraction method followed by the identification of its bioactive components. GSEx-CHNPs were synthesized by ionic gelation method and physically characterized then their activities were examined in vitro. The experimental groups, included immunocompetent and immunosuppressed groups, was treated with NPs for 14 days post infection (PI). The results showed the presence of many phenolic compounds in the GSEx. GSEx-CHNPs significantly improved the loss in animals body weight, cleared the infection and amolerated the serum cytokines levels. GSEx-CHNPs showed anti-cryptosporidial activity especially in immunosuppressed mice model. Where, it amolerated the disturbance in the cytokine profile leading to an anti-inflammatory response.
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    Preparation of bee venom-loaded chitosan nanoparticles for treatment of streptozotocin-induced diabetes in male Sprague Dawley rats
    (Beni-Suef University Journal of Basic and Applied Sciences, 2024) Alyaa Farid; Adham Mohamed; Ayten Ahmed; Farah Mehanny; Gehan Safwat
    Background Diabetes mellitus (DM) can be defned as an increase in the blood sugar level and a disturbance in protein, fat and carbohydrate metabolism. Bee venom (BV) is useful for treating and preventing diabetic rats’ histological and biochemical problems. Although the medical advantages of BV have been identifed, its safety has remained a substantial barrier for its application. Consequently, the goal of our work was to prepare bee venom-loaded chitosan (BV-CS) nanoparticles (NPs), which would then be physically characterized. This was followed by examining the efect of the synthetized BV-CS NPs on oxidation, infammation and coagulation in vitro. In diabetic rats’ model [induced by streptozotocin (STZ)], the produced BV-CS NPs were tested as an anti-diabetic medication. Results In vivo testing on pancreatic tissue homogenates showed that BV-CS NPs have antioxidant and antiinfammatory properties. The results showed that BV-CS NPs can be used as a safe and efcient therapy for diabetes. Up to a concentration of 250 µg/ml, the generated NPs demonstrated potential antioxidant, membrane stabilizing, and non-cytotoxic capabilities. Our fndings indicated that the administration of BV-CS NPs signifcantly controlled blood glucose levels and metabolic abnormalities that accompanied diabetes induction. Conclusions BV-CS NPs were successful in treating STZ-induced diabetes in rats, stimulated insulin secretion and were safe to be used in vivo. Key points 1. BV-CS NPs demonstrated potential in vitro antioxidant and non-cytotoxic capabilities. 2. BV-CS NPs increased insulin secretion and decreased blood sugar level. 3. BV-CS NPs reduced oxidative stress and infammation in vivo.