Browsing by Author "Ali M."

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    Design and synthesis of thiazol derivatives with biological evaluations as antitumor agents
    (Bentham Science Publishers B.V., 2015) El-Sharkawy K.A.; El-Brrati M.M.A.; Ghardaly I.A.; Ali M.; Mohareb, R.M., Department of Chemistry, Faculty of Science, Cairo University, Giza, Egypt; Abdo, N.Y.M., Chemistry Department, Faculty of Education, Alexandria University, Alexandria, 21526, Egypt; El-Sharkawy, K.A., Department of Pharmaceutical Chemistry, College of Pharmacy, Jazan University, P.O. Box 114, Jazan, 45142, Saudi Arabia, Department of Chemistry, Faculty of Biotechnology, October University for Modern Sciences and Arts (MSA), El-Wahat Road, 6th October City, Cairo, Egypt
    Background: Among the wide range of heterocycles, tetrahydrobenzothienopyridine derivatives acquired a special attention due to their wide range of pharmacological activities especially the therapeutic activities. Many pharmacological drugs containing the thiophene nucleus were known in the market. Method: A series of tetrahydrobenzothienopyridine derivatives were synthesized from the reaction of 2-amino- 3-benzoyl-4,5-dihydrobenzo[b]thiophen-6(7H)-one, synthesized and used for further heterocyclization reactions through reaction with different reagents. Results: Antiproliferative evaluations and c-Met kinase, Pim-1 kinase inhibitions were performed where some compounds revealed high activities. Conclusion: The inhibition of the newly synthesized compounds towards c-Met kinase, the five c-Metdependent cancer cell lines (A549, HT-29, MKN-45, U87MG, and SMMC-7721) and one c-Met-independent cancer cell line (H460) were investigated using foretinib as a standard drug. The results showed that compounds 6b, 7e, 9b, 9e, 16c and 20d were more active than foretinib. Furthermore, compounds 6b, 13b, 16b and 16c were selected to examine their Pim-1 kinase inhibition activity, where compounds 16b and 16c were of high potencies with IC50 values of 0.28 and 0.32 ?M, while compounds 6b and 13b were less effective (IC50 > 10 ?M). � 2018 Bentham Science Publishers.
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    In situ distribution of hepatitis C virus (HCV) RNA in the liver: Relationship to histopathology and serum HCV-RNA levels
    (2008) Helal T.E.-S.; Hassan M.I.; Ali M.; El-Ganzuri; Ahmed A.M.; Ezzelarab S.; Pathology Department; Faculty of Medicine; Ain Shams University; Cairo; Egypt; Department of Biochemistry; Faculty of Medicine; Cairo; Egypt; Lecturer of Cell Biology; Faculty of Dentistry; MSA University; Cairo; Egypt; Early Cancer Detection Unit; Faculty of Science; Ain Shams University; Cairo; Egypt
    In situ hybridization (ISH) technique has been applied to 60 liver biopsies obtained from Egyptian patients with chronic HCV hepatitis in an attempt to: assess the tissue localization of HCV RNA in the liver and to investigate its relationship to the liver pathology, serum level of alanine transaminase (ALT) and viral load in the serum. No similar studies have been reported from Egypt, which is an area of high HCV prevalence. ISH labeling for HCV was observed in 24 of the 60 liver biopsies from HCV infected patients (40%) as compared to the complete negative ISH results in the 10 control liver biopsies from patients with non HCV hepatitis. ISH positivity was seen only in the liver cells and it was usually cytoplasmic. ISH results were not significantly related to the patients' age, sex, histologic features of HCV hepatitis, grade of disease activity, stage of fibrosis, serum ALT levels or serum HCV RNA. However, ISH was statistically correlated to the grade of liver cell dysplasia (r=0.49, P<0.0001). The latter was also significantly correlated to the level of HCV RNA in the serum as measured by branched DNA technique (r=0.75, P<0.0001). In conclusion, the present study suggests that the load of HCV RNA in the liver is not related to extent of liver injury, serum ALT levels or the viral load in the serum. More importantly, the significant correlation between the grade of dysplasia and both the tissue and serum load of the virus, leads to the assumption that HCV has a direct carcinogenic effect on liver cells. Undoubtedly, these results are encouraging to plan for more large-scale studies on HCV hepatitis which is endemic in Egypt.