Browsing by Author "Afify, Fatma A"

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    Emergence of carbapenem resistant gram-negative pathogens with high rate of colistin resistance in Egypt: A cross sectional study to assess resistance trends during the COVID-19 pandemic
    (Academy of Scientific Research and Technology, 2024-03) Afify, Fatma A; Shata, Ahmed H; Aboelnaga, Nirmeen; Osama, Dina; Elsayed, Salma W; Saif, Nehal A; Mouftah, Shaimaa F; Shawky, Sherine M; Mohamed, Ahmed A; Loay, Omar; Elhadidy, Mohamed
    The current study investigated the temporal phenotypic and genotypic antimicrobial resistance (AMR) trends among multi‐drug resistant and carbapenem‐resistant Klebsiella pneumoniae, Acinetobacter baumannii, and Pseudomonas aeruginosa recovered from Egyptian clinical settings between 2020 and 2021. Bacterial identification and antimicrobial sensitivity of 111 clinical isolates against a panel of antibiotics were performed. Molecular screening for antibiotic resistance determinants along with integrons and associated gene cassettes was implemented. An alarming rate (98.2%) of these isolates were found to be phenotypically resistant to carbapenem. Although 23.9 % K. pneumoniae isolates were phenotypically resistant to colistin, no mobile colistin resistance (mcr) genes were detected. Among carbapenem‐resistant isolates, blaNDM and blaOXA‐48‐like were the most prevalent genetic determinants and were significantly overrepresented among K. pneumoniae. Furthermore, 84.78% of K. pneumoniae isolates co‐produced these two carbapenemase genes. The plasmid‐ mediated quinolone resistance genes (qnrS and qnrB) were detected among the bacterial species and were significantly more prevalent among K. pneumoniae. Moreover, Class 1 integron was detected in 82% of the bacterial isolates. This study alarmingly reveals elevated resistance to last‐resort antibiotics such as carbapenems as well as colistin which impose a considerable burden in the health care settings in Egypt. Our future work will implement high throughput sequencing‐based antimicrobial resistance surveillance analysis for characterization of novel AMR determinants. This information could be applied as a step forward to establish a robust antibiotic stewardship program in Egyptian clinical settings, thereby addressing the rising challenges of AMR.