Browsing by Author "Abdelkawy M."

Filter results by typing the first few letters
Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Development and validation of simultaneous spectrophotometric and TLC-spectrodensitometric methods for determination of beclomethasone dipropionate and salbutamol in combined dosage form
    (Elsevier, 2014) Samir A.; Lotfy H.M.; Salem H.; Abdelkawy M.; Analytical Chemistry Department; Faculty of Pharmacy; Cairo University; Egypt; Analytical Chemistry Department; Faculty of Pharmacy; October University for Modern Sciences and Arts; Egypt
    Spectrophotometric and TLC-spectrodensitometric methods were developed and validated for the simultaneous determination of beclomethasone dipropionate (BEC) and salbutamol (SAL). The spectrophotometric methods include dual wavelength, ratio difference, constant center coupled with a novel method namely, spectrum subtraction and mean centering with mean percentage recoveries and RSD 99.72 � 1.07 and 99.70 � 1.12, 100.25 � 1.12 and 99.89 � 1.12, 99.66 � 1.85 and 99.19 � 1.32, 100.74 � 1.26 and 101.06 � 0.90 for BEC and SAL respectively. The TLC-spectrodensitometric method was based on separation of both drugs on TLC aluminum plates of silica gel 60 F254, using benzene: methanol: triethylamine (10:1.5:0.5 v/v/v) as a mobile phase, followed by densitometric measurements of their bands at 230 nm. The mean percentage recoveries and RSD were 99.07 � 1.25 and 101.35 � 1.50 for BEC and SAL respectively. The proposed methods were validated according to ICH guidelines and were applied for the simultaneous analysis of the cited drugs in synthetic mixtures and pharmaceutical preparation. The methods were found to be rapid, specific, precise and accurate and can be successfully applied for the routine analysis of BEC and SAL in their pharmaceutical formulation with no need for prior separation. The results obtained were statistically compared to each other and to that of the reported HPLC method. The statistical comparison showed that there is no significant difference regarding both accuracy and precision. � 2014 Elsevier B.V. All rights reserved.
  • Thumbnail Image
    Item
    Spectrophotometric methods for simultaneous determination of betamethasone valerate and fusidic acid in their binary mixture
    (Elsevier, 2015) Lotfy H.M.; Salem H.; Abdelkawy M.; Samir A.; Analytical Chemistry Department; Faculty of Pharmacy; Cairo University; Kasr El-Aini Street; Cairo; 11562; Egypt; Pharmaceutical Analytical Chemistry Department; Faculty of Pharmacy; Deraya University; Minia; Egypt; Analytical Chemistry Department; Faculty of Pharmacy; MSA University; 6th October City; Egypt
    Five spectrophotometric methods were successfully developed and validated for the determination of betamethasone valerate and fusidic acid in their binary mixture. Those methods are isoabsorptive point method combined with the first derivative (ISO Point - D1) and the recently developed and well established methods namely ratio difference (RD) and constant center coupled with spectrum subtraction (CC) methods, in addition to derivative ratio (1DD) and mean centering of ratio spectra (MCR). New enrichment technique called spectrum addition technique was used instead of traditional spiking technique. The proposed spectrophotometric procedures do not require any separation steps. Accuracy, precision and linearity ranges of the proposed methods were determined and the specificity was assessed by analyzing synthetic mixtures of both drugs. They were applied to their pharmaceutical formulation and the results obtained were statistically compared to that of official methods. The statistical comparison showed that there is no significant difference between the proposed methods and the official ones regarding both accuracy and precision. � 2015 Elsevier B.V. All rights reserved.