Browsing by Author "Abdelhamid, A.M"

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    The cardioprotective effect of astaxanthin against isoprenaline-induced myocardial injury in rats: Involvement of TLR4/NF-κB signaling pathway
    (Verduci Editore s.r.l, 2021) Zaafan, M.A; Abdelhamid, A.M
    OBJECTIVE: Cardiovascular diseases (CVDs) are a major cause of morbidity and mortality around the world. Nuclear transcription factor kappa B (NF-κB) represents a factor that plays a major role in the pathogenesis of CVDs. The current study aims to investigate the modulatory effects of astaxanthin and its molecular mechanisms in rats with isoprenaline-induced myocardial infarction. MATERIALS AND METHODS: Rats were pretreated with astaxanthin daily for 14 days prior to inducing myocardial infarction with isoprenaline in the final two days. Blood and heart tissue samples were collected 24 hours after the last dose of isoprenaline was injected for biochemical and histological analysis. RESULTS: Isoprenaline-induced myocardial injury was demonstrated with histopathological examination of heart tissue and the significantly elevated serum troponin-I. Isoprenaline caused an increase in oxidative stress and a decrease in antioxidants. Toll-like receptor-4 (TLR4), NF-κB and tumor necrosis factor-α (TNF-α) expression levels were significantly higher in infarcted rats. Astaxanthin pretreatment had a significant preventive effect on all of the biochemical and molecular parameters tested in myocardial infarcted rats. CONCLUSIONS: Astaxanthin’s cardioprotective effect has been linked to the inhibition of the TLR4/NF-κB signaling pathway. This inhibits the release of inflammatory cytokines, which can cause myocardial cell death. Because of its antioxidant and anti-inflammatory properties, astaxanthin is a promising cardioprotective agent. © 2021 Verduci Editore s.r.l. All rights reserved.
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    Circulating microRNA-944 and its target gene EPHA7 as a potential biomarker for colorectal cancer
    (Taylor and Francis Ltd, 5/18/2020) Shaker, Olfat G; Ayeldeen, G; Abdelhamid, A.M
    MicroRNAs (miRNAs) have critical roles in colorectal cancer (CRC) tumorigenesis and development. It has been reported that Eph receptor A7 (EphA7) was a potential target of miR-944 which is transcriptionally activated in cancer. The aim of this study was to explore the expression profile of miR-944 and its target gene EPHA7 in the serum of Egyptian CRC patients. 150 CRC patients, 50 adenomatous polyps (AP) patients, and 100 healthy controls were included. Serum miR-944 was downregulated (0.304 ± 0.0512) while serum EPHA7 was upregulated (3.163 ± 0.610) in CRC and AP patients versus controls and discriminated aganst these groups by Receiver operating characteristic curve (ROC) analysis. miR-944 presented the highest diagnostic accuracy for CRC patients from control (AUC = 0.90). Moreover obvious prognostic power in distinguishing AP from CRC (AUC = 0.87). In conclusion, miR-944 and EPHA7 are potential genetic markers of CRC predisposition and novel potential non-invasive diagnostic biomarkers for CRC. © 2020, © 2020 Informa UK Limited, trading as Taylor & Francis Group