Browsing by Author "Abdel-Hafiz S.M."

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    Evaluation of osteopontin as a biomarker in Hepatocellular carcinomas in Egyptian patients with chronic HCV cirrhosis
    (Asian Pacific Organization for Cancer Prevention, 2018) Abdel-Hafiz S.M.; Hamdy H.E.; Khorshed F.M.; Aboushousha T.S.; Safwat G.; Saber M.A.; Seleem M.; Soliman A.H.; Theodore Bilharz Research Institute (TBRI); Egypt; Faculty of Biotechnology; October University of Modern Sciences and Arts; Egypt; Theodore Bilharz Research Institute El-Warrak; Embaba Giza; Egypt; National Hepatology and Tropical Medicine Institute (NHTMRI); Egypt; National Cancer Institute; Cairo University; Cairo; Egypt
    Background: Hepatocellular carcinoma (HCC) is a high incidence disease in Egypt with a poor prognosis and survival. Biomarkers are important for diagnosis of HCC at an early stage. Osteopontin (OPN), a glycoprotein secreted by macrophages, osteoblasts, and T cells, is also highly expressed in a variety of tumors, such as examples in the breast, colon, and stomach. The present study aimed to correlate the serum level of OPN in HCV-positive hepatocellular carcinoma patients, with OPN expression in tumor and non-tumor liver tissues in order to identify its efficacy as a biomarker for diagnosis. Material and Methods: Out of total of 146 patients, 80 were selected for inclusion in the study. Blood samples as well as specimens of tumor and non-tumor liver tissue were collected. In addition, blood samples from 20 healthy volunteers were obtained as controls. Serum OPN and alpha-fetoprotein (AFP) were evaluated by ELISA for HCC and control groups. OPN and AFP gene expression were examined by real-time PCR, after homogenization and DNA extraction from serum samples and liver tissues. Results: It was found that serum OPN levels were significantly higher in the HCC group compared to normal group (P=0.009), with a strong positive correlation with AFP expression. However, there was no significant difference between OPN expression in tumor and non-tumor liver tissue. Conclusion: Serum OPN is highly suggested to be a professional candidate for HCC early diagnosis, with a diagnostic ability and accuracy equal or higher than for AFP. � 2017, Asian Pacific Organization for Cancer Prevention.