Browsing by Author "Abdel Sattar, Essam"

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    Application of a comprehensive metabolomics approach for the selection of flaxseed varieties with the highest nutritional and medicinal attributes
    (6/15/2021) Salem, Mohamed A; Ezzat, Shahira M; Giavalisco, Patrick; Abdel Sattar, Essam; El Tanbouly, Nebal
    Flaxseed is considered an indispensable generally recognized as safe (GRAS) source of polyphenolic lignans, poly- unsaturated fatty acids (PUFA), fibers as well as minerals and vitamins. The metabolite content of flaxseed reflects its nutritional and medicinal value. Therefore, the selection of flaxseed variety for food industry is dependent on its metabolome. A metabolomics approach based on liquid or gas chromatography coupled to mass spectrometry has been applied to discriminate different flaxseed cultivars that are commercially available in Egypt. The available Sakha cul- tivars were subjected to a comprehensive metabolomics and lipidomics approach for investigation of their metabolomes. Our results showed that among the screened cultivars, Sakha 6, with its yellow-colored testa, showed marked metabolic discrimination. This yellow cultivar showed high accumulation of essential amino acids. Additionally, the oil of this cultivar accumulated the highest content of the two essential PUFA: alpha-linolenic acid (an u-3 fatty acid) and linoleic acid (an u-6 fatty acid). Interestingly, the content of the main antinutritional cyanogenic glycosides such as linustatin and neolinustatin was lower, while, the content of medicinally-important secondary metabolites was higher in Sakha 6 cultivar. These results support the use of this cultivar for human consumption owing to its high nutritional and me- dicinal value
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    Evaluation of the Anti-inflammatory and Antioxidant Activities of Selected Resin Exudates
    (Springer, 2020-07) Ali, Dalia E.; Abdelrahman, Rehab S.; El Gedaily, Rania A,; Ezzat, Shahira M; Meselhy, Meselhy R.; Abdel Sattar, Essam
    Plant resins are reported to have high medicinal values due to their content of bioactive metabolites. Few reports were found in the last two decades concerning the chemistry and anti- inflammatory activity of the resins belonging to Eucalyptus and Araucaria genera. Therefore, the exudate resins of Eucalyptus maculata, Araucaria excelsa and Araucaria bidwillii were evaluated for their phenolic and flavonoid content, together with their possible anti- inflammatory potential via carrageenan-induced paw edema in rats at the doses of 100, 200, and 400 mg/kg. The methanol extract of E. maculata (MEME) kino resin was the richest in phenolics (572.82 ± 1.75 µg GAE/mg extract), and flavonoids (11.60 ± 0.01 µg QE/mg extract). The MEME (400 mg/kg) showed the most significant percentage inhibition of paw oedema (50.91%), compared to indomethacin (66.11%). The extract also decreased serum malondialdehyde (MDA) (42%), nuclear factor kappa B (NF-κB) (42.7%), tumor necrosis factor-alpha (TNF-α) (40.5%), cyclooxygenase-2 (COX-2) (42%) and nitric oxide (NO) (23%). In addition, it increased antioxidant enzymes; reduced glutathione (GSH) (2.6-fold) and superoxide dismutase (SOD) (1.3-fold), compared to control group. Moreover, it displayed a profound antioxidant property with IC50 of 6.83 ± 0.77μg/mL compared to trolox (IC50 21.18 ± 0.59 μg/mL) using DPPH assay. The methanol extract of E. maculata resin was standardized by HPLC to contain 68.21 mg of 7-O-methyl aromadendrin (MA)/g extract. These findings suggest that E. maculata kino resin is a potent antioxidant with potential therapeutic efficacy for treating inflammation.