Browsing by Author "Abdel Latif, Yasmin"

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Dynamic expression of H19 and MALAT1 and their correlation with tumor progression biomarkers in a multistage hepatocarcinogenesis model
(John Wiley and Sons Ltd, 2023-03) El-Daly, Sherien M; El-Bana, Mona A; Abd El-Rahman, Sahar S; Abdel Latif, Yasmin; Medhat, Dalia
Hepatocellular carcinoma (HCC) progresses sequentially in a stepwise pattern. Long noncoding RNA (lncRNA) can regulate the complex cascade of hepatocarcinogenesis. Our study aimed to elucidate the expression profile of H19 and MALAT1 during the different stages of hepatocarcinogenesis and the correlation between H19 and MALAT1 with the genes implicated in the carcinogenesis cascade. We employed a chemically induced hepatocarcinogenesis murine model to mimic the successive stages of human HCC development. Using real-time PCR, we analyzed the expression patterns of H19 and MALAT1, as well as the expression of biomarkers implicated in the Epithelial-Mesenchymal transition (EMT). The protein expression of the mesenchymal marker vimentin was also evaluated using immunohistochemistry in the stepwise induced stages. The histopathological evaluation of the liver tissue sections revealed significant changes during the experiment, with HCC developing at the final stage. Throughout the stages, there was a dynamic significant increase in the expression of H19 and MALAT1 compared to the normal control. Nevertheless, there was no significant difference between each stage and the preceding one. The tumor progression biomarkers (Matrix Metalloproteinases, vimentin, and β-catenin) exhibited the same trend of steadily increasing levels. However, in the case of Zinc finger E-box-binding homeobox 1 and 2 (ZEB1 and ZEB2), the significant elevation was only detected at the last stage of induction. The correlation between lncRNAs and the tumor progression biomarkers revealed a strong positive correlation between the expression pattern of H19 and MALAT1 with Matrix Metalloproteinases 2 and 9 and vimentin. Our findings imply that genetic and epigenetic alterations influence HCC development in a stepwise progressive pattern.
Irisin, an Exercise Stimulated Hormone as a Metabolic Regulator in Metabolic Syndrome in Obese Rats
(NIDOC (Nat.Inform.Document.Centre), 2022-03) Rasheed, Wafaa I; Elias, Tahany R; Ashour, Magdy N; Agaibyi, Mervat H; Al Wassif, Maha; Youness, Eman R; Abdel Latif, Yasmin; Nazih, Noha
Irisin plays an important role in energy metabolism and regulation of metabolic diseases as obesity and diabetes. This study aimed to evaluate the modulatory effect of physical exercise on the irisin as an exercise stimulated hormone and other biochemical parameters in obese rats suffering from metabolic syndrome (MS). Sixty male albino rats were randomly divided into 4 groups (15 in each): group I (control) healthy rats were fed on standard (St.) diet, group II (contol+ex) in which rats were fed on St. diet and made exercise (ex.), group III: rats were fed on high carbohydrate high fat (HCHF) diet induced obesity in rats. Group IV (HCHF+ex.): rats also fed on HCHF diet and made exercise for 20 weeks. Serum glucose, insulin, lipid profile, irisin, Peroxisome proliferator-activated receptor -alpha (PPAR-𝛼) and Interleukin 6 (IL-6) were measured. Histopathological examination of liver, pancreas and muscle was done. Our results revealed that glucose, lipid profile except HDL-C and IL-6 significantly increased, whereas a remarkable decrease in serum level of irisin and PPAR-α in HCHF group comparing to the control group. After swimming, all parameters were improved. Conclusion: swimming exercise has an efficient effect in improving all biochemical parameters of MS due to releasing of irisin which has regulatory mechanisms with respect to metabolic metabolism -associated health issues.
Processed Cheeses Fortified by Laurus nobilis L. Extract Nanoemulsion Ameliorate Hyperhomocysteinemia in Ehrlich Ascites Carcinoma Model
(NIDOC (Nat.Inform.Document.Centre), 2023-02) Hussein, Jihan; El- Bana, Mona A; Abdel Latif, Yasmin; El-sayed, Samah M; Youssef, Ahmed M; El-Naggar, Mehrez E; Medhat, Dalia
We aimed to evaluate the protective effect of processed cheeses fortified by Laurus nobilis L. extract nanoemulsion against hyperhomocysteinemia in Ehrlich ascites carcinoma (EAC) model. Laurus nobilis L. extract nanoemulsion was prepared via spontaneous emulsification procedure. Then, nanoemulsion was added by different loadings to supplement processed cheeses. The morphology of Laurus nobilis L. extract nanoemulsion was detected using transmission electron microscopy (TEM). Female Swiss albino mice received processed cheese for two weeks then inoculated once with EAC cells. After the end of the experiment, blood samples were collected for determination of serum superoxide dismutase (SOD) , thiobarbituric acid reactive substances (TBARS), B-cell lymphoma 2 (Bcl-2), matrix metallopeptidase-9 (MMP-9), Tumor necrosis factor-α (TNF-α), and homocysteine (Hcy). Results revealed that the formation of Laurus nobilis L. extract nanoemulsion in spherical shape in size around 50 -120 nm. Mice inoculated with EAC cells showed significant increase in serum Hcy, TNF-α, TBARS, and MMP-9 levels while there was a significant decrease in SOD activity and Bcl-2 level. Pretreatment with different concentrations of Laurus nobilis L. extract significantly attenuated oxidative stress, inflammation, and induced apoptosis compared to EAC group.