Browsing by Author "Abd El-Rahman, Ola A."

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    Co-Existence of Carbapenemase-Encoding Genes in Acinetobacter baumannii from Cancer Patients
    (ADIS, 11/12/2020) Wasfi, Reham; Rasslan, Fatma; Hassan, Safaa S.; Ashour, Hossam M.; Abd El-Rahman, Ola A.
    Introduction: Acinetobacter baumannii is an opportunistic pathogen, which can acquire new resistance genes. Infections by carbapenem-re- sistant A. baumannii (CRAB) in cancer patients cause high mortality. Methods: CRAB isolates from cancer patients were screened for carbapenemase-encoding genes that belong to Ambler classes (A), (B), and (D), followed by genotypic characterization by enterobacterial-repetitive-Intergenic- consensus–polymerase chain reaction (ERIC–PCR) and multilocus-sequence-typing (MLST). Results: A total of 94.1% of CRAB isolates co- harbored more than one carbapenemase-en- coding gene. The genes blaNDM, blaOXA-23-like, and blaKPC showed the highest prevalence, with rates of 23 (67.7%), 19 (55.9%), and 17 (50%), respectively. ERIC-PCR revealed 19 patterns (grouped into 9 clusters). MLST analysis identi- fied different sequence types (STs) (ST-268, ST- 195, ST-1114, and ST-1632) that belong to the highly resistant easily spreadable International clone II (IC II). Genotype diversity indicated the dissemination of carbapenem-hydrolyzing, b-lactamase-encoding genes among genetically unrelated isolates. We observed a high preva- lence of metallo-b-lactamase (MBL)-encoding genes (including the highly-resistant blaNDM gene that is capable of horizontal gene transfer) and of isolates harboring multiple carbapene- mase-encoding genes from different classes. Conclusion: The findings are alarming and call for measures to prevent and control the spread of MBL-encoding genes among bacteria causing infections in cancer patients and other immunocompromised patient populations.