Browsing by Author "A. Diab, Ayman"

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Drought - inducible genes and differentially expressed sequence tags associated with components of drought tolerance in durum wheat
(Scientific Research and Essay, 2008) A. Diab, Ayman; V. Kantety, R.; Z. Ozturk 3, N.; Benscher, D.; M. Nachit, M.; E. Sorrells, M.
Drought stress is one of the most important environmental factors reducing cereal yields. The genetic and physiological bases of drought tolerance in durum wheat was investigated by QTL mapping and by mapping candidate genes derived from differentially expressed genes and previous investigations. A recombinant inbred population derived from a cross between two durum (Triticum turgidum L. var durum) parents Jennah Khetifa and Cham1 that exhibit contrasting traits for drought tolerance was employed. Twelve known genes and 103 differentially expressed sequence tags (dESTs) were surveyed and 55 detected polymorphism between the two parental lines. In total, 162 loci including 6 known genes, 37 dESTs, and an additional 119 markers that were unlinked in the previous map have been merged with 306 previously mapped markers to produce a new map with 468 loci. Single point analysis and composite interval mapping were used to identify the genomic regions controlling traits related to drought stress. Significant QTL were identified for canopy temperature, photosynthesis-related parameters and water status index. One hundred and seventy eight markers, including 6 candidate genes and 19 differentially expressed sequences were associated with QTL for drought tolerance traits. The results indicate that there is considerable potential for improving drought tolerance of durum wheat by using marker-assisted selection
Identification of Chromosomal Regions and Genetic Contributions of Genes Controlling Yield and Other Agronomic Traits in Durum Wheat Grown under Different Egyptian Environmental Conditions
(World Journal of Agricultural Sciences, 2007) A. Diab, Ayman; H. Fahmy, Ashraf; S. Hassan, Osama; M. Nachit, M.; A. Momtaz, Osama
A better understanding of the genetics of complex traits, such as yield, may be achieved by using molecular tools. Molecular markers provide a rapid approach to breeding for desired agronomic traits. To use them, it is necessary to determine the linkage between quantitative trait loci (QTLs) and such markers. This study was conducted to estimate the number and effect of alleles and the chromosomal locations of QTLs responsible for yield and some agronomic traits in durum wheat. A recombinant inbred population derived from a cross between two durum (Triticum turgidum L. var durum) parents Jennah Khetifa and Chaml was characterized for molecular markers and traits measured in different Egyptian environments. The environmental and genotypic effects on the measured traits were determined. Single point analysis (using Qgene) and conposite interval mapping (using QTL cartographer) were used to identify …