Abstract:
Context: Despite the traditional use of Bergia ammannioides Henye ex Roth. (Elatinaceae) for
the treatment of wounds in India, there is a scarcity of scientific data supporting this use.
Objective: The objective of this study is to assess wound-healing potentiality of the plant,
to study pharmacological activities that may contribute in eliminating wound complications,
and to investigate the biologically active fractions.
Material and methods: The ethanolic extract (EtOH) of the aerial parts was fractionated to
obtain n-hexane (HxFr), chloroform (ClFr), ethyl acetate (EtFr), and n-butanol (BuOH) fractions.
EtOH and its fractions were formulated in strength of 5 and 10% w/w ointment and tested
for wound-healing activity using the excision model. The topical anti-inflammatory, in vitro
antioxidant, and antibacterial activities were evaluated. HxFr and EtFr were chemically
investigated to isolate their constituents.
Results: Application of EtOH, HxFr, and EtFr (10% w/w ointments) leads to 71.77, 85.62,
and 81.29% healing of the wounds with an increase in the collagen content. HxFr had the
strongest anti-inflammatory (64.5% potency relative to Voltaren) and antibacterial activity
(MIC ¼ 104 mg/ml against Staphylococcus aureus), while EtFr showed the strongest antioxidant
activity against DPPH, ABTS+
, and super oxide radical with an IC50 value of 10.25 ± 0.01,
66.09 ± 0.76, and 167.33 ± 0.91 mg/ml, respectively. b-Sitosterol, lupeol, cyclolaudenol, and
cycloartenol were isolated from HxFr. Quercetin, ellagic acid, kaempferol-3-O-a-L-rhamnoside,
and quercetin-3-O-a-L-rhamnoside were isolated from EtFr.
Discussion and conclusion: Our study presents scientific evidence for the efficacy of
B. ammannioides in enhancing wound healing, and the first isolation of cyclolaudenol and
cycloartenol from Bergia
