Abstract:
Nuts are very useful natural plants having different shapes and biological activities. Literature reported that peanut oil has emollient, antioxidant, antiaging and anti-inflammatory activities on the skin. Also, hazelnut oil was reported to have hydrating effect on the skin, improves collagen production and reduces fine lines by its anti-wrinkle activity and reduces dark spots by anti-hyperpigmentation effect. Almond oil is rich with vitamin E and zinc thus enhancing the skin tone, decreasing the damaging effect of sun lights, aging signs, scars, and acne. Walnut oil has antimicrobial, anti-inflammatory, brightening and antioxidant activities that help in treating skin infections, acne and protecting skin from clogging of pores. Finally, walnut has anti-aging effect on skin by inhibiting collagenase and elastase enzymes. Thus, suggesting these oils to be a promising oil in cosmetic skin formulations. Nuts oils are also rich sources for phenolic compounds, fatty acids, and sterols. The aim of work is the extraction of the oil of the edible parts of four selected nuts (walnut, peanuts, hazelnuts, and almond) and determination of their chemical composition using GC/MS, as well as, studying the in vitro effect of oils on skin as anti-aging and anti-wrinkles activities to provide effective cosmetic skin formulations of low price with lower side effects from natural source. The result of in vitro assay showed that walnut oil (S4) showed highest activity in procollagen type 1 C peptide with maximum inhibition of tyrosinase and lowest level of melanin followed by almond oil (S1) then hazelnut oil (S2) and peanut oil (S3). While almond oil (S1) showed highest level of hyaluronic acid and collagen content with lowest level of metalloproteinases followed by walnut oil (S4) then hazelnut oil (S2) and peanut oil (S3). Finally almond oil (S1) showed lowest level of MTT cytotoxicity assay followed by peanut oil (S3) then walnut oil (S4) and hazelnut oil (S2). In addition to that the In vivo results show that almond oil has higher levels of Procollagen, Collagen, Hyaluronic, Tissue inhibitor of metalloproteinases 1 (TIMP), and Glutathione (GSH). While walnut oil has high level of nuclear factor kappa B (NFKB), Interleukin Beta (IL1B), Tyrosinase, Metalloproteinases (MMP1&9), and Malondialdehyde (MDA).
