Abstract:
Aim
Poultry are great sources of protein and other important nutrients for our body. So, poultry
should be included as a part of a balanced diet. Residues of veterinary drugs in poultry meat have
serious health effects on humans (e.g., antimicrobial resistance, carcinogenicity, and
hypersensitivity), making controlling veterinary drug residues an important parameter in ensuring
consumer protection. Hence, it is important to study the withdrawal time of these drugs in chicken
tissues.
Materials and Methods
This work was performed to quantitatively determine a co-formulated veterinary
formulation namely, Coccimix®, consisting of two sulfonamides (Sulfadimidine sodium and
Sulfaquinoxaline sodium), in addition to Diaveridine, and Vitamin K3 in different tissues of broiler
chickens including muscles (breast and thigh) and liver. The chickens were treated with the
recommended dose of Coccimix® formulation. The analysis was done by solvent extraction for
clean-up of samples from the tissue matrix, followed by HPLC-MS/MS method using Agilent C18
4.6*50 mm 2.7-Micron as a stationary phase and a mobile phase of acetonitrile: 0.1% formic acid
in water (85: 15 v/v) with positive electrospray ionization.
Results
The proposed method was validated according to FDA guidance for bioanalytical method
validation. The residues of the four components declined to reach levels lower than MRLs over a
period of 7 days. Both sulfonamides and Diaveridine were found to be more persistent in liver
tissues than muscle tissues.
Conclusion
It is recommended to monitor the withdrawal (elimination), especially for antibiotics,
before marketing to ensure the hygienic suitability of broilers edibles for safe human consumption
