Abstract:
The Kidneys remove toxins from the blood and move waste products into the urine. However, the
accumulation of toxins and fuids in the body leads to kidney failure. For example, the overuse of
acrylamide and titanium dioxide nanoparticles (TiO2NPs) in many food and consumer products
increases human exposure and risks; however, there are almost no studies available on the efect
of TiO2NPs coadministration with acrylamide on the integrity of genomic and mitochondrial DNA.
Accordingly, this study was conducted to estimate the integrity of genomic and mitochondrial
DNA in the renal tissue of mice given acrylamide and TiO2NPs. To achieve this goal, mice were
administrated orally TiO2NPs or/and acrylamide at the exposure dose levels (5 mg/kg b.w) and (3 mg/
kg b.w), respectively, fve times per week for two consecutive weeks. Concurrent oral administration
of TiO2NPs with acrylamide caused remarkable elevations in the tail length, %DNA in tail and
tail moment with higher fragmentation incidence of genomic DNA compared to those detected
in the renal tissue of mice given TiO2NPs alone. Simultaneous coadministration of TiO2NPs with
acrylamide also caused markedly high elevations in the reactive oxygen species (ROS) production
and p53 expression level along with a loss of mitochondrial membrane potential and high decreases
in the number of mitochondrial DNA copies and expression level of β catenin gene. Therefore,
from these fndings, we concluded that concurrent coadministration of acrylamide with TiO2NPs
augmented TiO2NPs induced genomic DNA damage and mitochondrial dysfunction through
increasing intracellular ROS generation, decreasing mitochondrial DNA Copy, loss of mitochondrial
membrane potential and altered p53 and β catenin genes expression. Therefore, further studies are
recommended to understand the biological and toxic efects resulting from TiO2NPs with acrylamide
coadministration.
