dc.contributor.author |
Mokhtar, Fatma A |
|
dc.contributor.author |
Selim, Nabil M |
|
dc.contributor.author |
Elhawary, Seham S |
|
dc.contributor.author |
Abd El Hadi, Soha R |
|
dc.contributor.author |
Hetta, Mona H |
|
dc.contributor.author |
Albalawi, Marzough A |
|
dc.contributor.author |
Shati, Ali A |
|
dc.contributor.author |
Alfaifi, Mohammad Y |
|
dc.contributor.author |
Elbehairi, Serag Eldin I |
|
dc.contributor.author |
Fahmy, Lamiaa I |
|
dc.contributor.author |
Ibrahim, Rana M |
|
dc.date.accessioned |
2022-11-30T07:26:36Z |
|
dc.date.available |
2022-11-30T07:26:36Z |
|
dc.date.issued |
2022-11 |
|
dc.identifier.other |
https://doi.org/10.3390/ ph15111354 |
|
dc.identifier.uri |
http://repository.msa.edu.eg/xmlui/handle/123456789/5264 |
|
dc.description.abstract |
Annona glabra L. (AngTE) and Annona squamosa L. (AnsTE) fruits have been widely used
in cancer treatment. Accordingly, their extracts were used to synthesize silver nanoparticles via a
biogenic route (Ang-AgNPs) and (Ans-AgNPs), respectively. Chemical profiling was established
using UPLC-QTOF-MS/MS. All species were tested for anticancer activity against human cervical
cancer cells (HeLa), prostate adenocarcinoma metastatic (PC3), and ovary adenocarcinoma (SKOV3)
using sulphorhodamine B assay. Apoptosis was determined using Annexin flow cytometry along
with cell cycle analysis and supported by a molecular docking. The antibacterial and synergistic
effect when combined with gentamicin were evaluated. A total of 114 compounds were tentatively
identified, mainly acetogenins and ent-kaurane diterpenes. AnsTE and Ans-AgNPs had the most
potent cytotoxicity on HeLa and SKOV3 cells, inducing a significant apoptotic effect against all tumor
cells. The AnsTE and Ans-AgNPs significantly arrested PC3, SKOV3, and HeLa cells in the S phase.
The nanoparticles demonstrated greater antibacterial and antifungal activities, as well as a synergistic
effect with gentamicin against P. aeruginosa and E. coli. Finally, a molecular docking was attempted to
investigate the binding mode of the identified compounds in Bcl-2 proteins’ receptor, implying that
the fruits and their nanoparticles are excellent candidates for treating skin infections in patients with
ovarian or prostatic cancer. |
en_US |
dc.description.uri |
https://www.scimagojr.com/journalsearch.php?q=17700156714&tip=sid&clean=0 |
|
dc.language.iso |
en_US |
en_US |
dc.publisher |
Multidisciplinary Digital Publishing Institute (MDPI) |
en_US |
dc.relation.ispartofseries |
. Pharmaceuticals;2022, 15, 1354 |
|
dc.subject |
Annona; UPLC-QTOF-MS/MS |
en_US |
dc.subject |
silver nanoparticles |
en_US |
dc.subject |
anticancer |
en_US |
dc.subject |
apoptosis |
en_US |
dc.subject |
antibacterial |
en_US |
dc.subject |
antifungal |
en_US |
dc.subject |
docking |
en_US |
dc.title |
Green Biosynthesis of Silver Nanoparticles Using Annona glabra and Annona squamosa Extracts with Antimicrobial, Anticancer, Apoptosis Potentials, Assisted by In Silico Modeling, and Metabolic Profiling |
en_US |
dc.type |
Article |
en_US |
dc.identifier.doi |
https://doi.org/10.3390/
ph1511135 |
|
dc.Affiliation |
October university for modern sciences and Arts MSA |
|