dc.contributor.author |
AL-Ghamdi, Maryam Abdu |
|
dc.contributor.author |
Alsulami, Rawyah Radi |
|
dc.contributor.author |
Bakkar, Ashraf |
|
dc.contributor.author |
Kumosani, Taha Abullah |
|
dc.contributor.author |
Barrbour, Elie Kamil |
|
dc.contributor.author |
Abulnaja, Khalid Omar |
|
dc.contributor.author |
Huwait, Etimad |
|
dc.contributor.author |
Moselhy, Said Salama |
|
dc.date.accessioned |
2022-11-11T06:27:34Z |
|
dc.date.available |
2022-11-11T06:27:34Z |
|
dc.date.issued |
2022-11 |
|
dc.identifier.other |
https://doi.org/10.1080/14786419.2022.2140803 |
|
dc.identifier.uri |
https://bit.ly/3fWPf2x |
|
dc.description.abstract |
Autophagy is a protective mechanism important in human dis-
eases as cancer. We evaluated the impact of khalas date extract
(KDE) (20-60 mg/mL) on cell viability, morphological changes,
DNA fragmentation and gene expression of LC3B-II associated
with autophagosome on HepG2 cell line. The GC/MS identification
of KDE showed its high content of flavonoids including quercetin,
myricetin, kaempferol and catechol. KDE reduced cell viability of
HepG2 with IC50 (31.52 mg/mL). Cells treated with KDE showed
two band of DNA fragments at (30 and 40 mg) indicating that
KDE induced DNA damage and apoptosis in HepG2. The analysis
RT-PCR data showed a 0.2-fold increase in the expression of LC3-
B in the cells treated with KDE versus control. We concluded that,
KDE flavonoids such as quercetin, myricetin kaempferol exhibited
anticancer properties manifested by inhibition of HepG2 cell via-
bility and induction of apoptosis and upregulation of the pro-
autophagy LC3-B gene.
ARTICLE HISTORY |
en_US |
dc.description.uri |
https://www.scimagojr.com/journalsearch.php?q=24819&tip=sid&clean=0 |
|
dc.language.iso |
en_US |
en_US |
dc.publisher |
Taylor and Francis Ltd. |
en_US |
dc.relation.ispartofseries |
NATURAL PRODUCT RESEARCH; |
|
dc.subject |
Khalas date |
en_US |
dc.subject |
flavonoids |
en_US |
dc.subject |
autophagy |
en_US |
dc.subject |
cytotoxicity |
en_US |
dc.subject |
HepG2 |
en_US |
dc.title |
Khalas date flavonoids inhibited cell viability, induced apoptosis and expression of the pro-autophagy LC3-B gene in human hepatocellular carcinoma cells (HepG2) |
en_US |
dc.type |
Article |
en_US |
dc.identifier.doi |
https://doi.org/10.1080/14786419.2022.2140803 |
|
dc.Affiliation |
October university for modern sciences and Arts MSA |
|