Abstract:
Autophagy is a protective mechanism important in human dis-
eases as cancer. We evaluated the impact of khalas date extract
(KDE) (20-60 mg/mL) on cell viability, morphological changes,
DNA fragmentation and gene expression of LC3B-II associated
with autophagosome on HepG2 cell line. The GC/MS identification
of KDE showed its high content of flavonoids including quercetin,
myricetin, kaempferol and catechol. KDE reduced cell viability of
HepG2 with IC50 (31.52 mg/mL). Cells treated with KDE showed
two band of DNA fragments at (30 and 40 mg) indicating that
KDE induced DNA damage and apoptosis in HepG2. The analysis
RT-PCR data showed a 0.2-fold increase in the expression of LC3-
B in the cells treated with KDE versus control. We concluded that,
KDE flavonoids such as quercetin, myricetin kaempferol exhibited
anticancer properties manifested by inhibition of HepG2 cell via-
bility and induction of apoptosis and upregulation of the pro-
autophagy LC3-B gene.
ARTICLE HISTORY
