Abstract:
Musa acuminata (MA) is a popular fruit peels in the world. Non-food parts of the plant have been
investigated for their antioxidant and anti-ulcerative colitis activity. Metabolomic approaches
were found to be informative as a screening tool. It discovered diferent metabolites depending on
statistical analysis. The antioxidant activity content was measured by colorimetric method. Seventy
six investigated metabolites were observed. The identities of some of these markers were confrmed
based on their MS2
fragmentation and NMR spectroscopy. These include: cinnamic acid and its dimer
2-hydroxy-4-(4-methoxyphenyl)-1H-phenalen-1-one beside; gallic acid and favonoids; quercetin,
quercetin-3-O-β-d-glucoside, luteolin-7-O-β-d-glucopyranoside. GC/MS analysis of MA peels essential
oil led to identifcation of 37 compounds. The leaves, pseudostem and fruit peels extracts were tested
for their safety and their anti-ulcerative colitis efcacy in rats. Rats were classifed into: normal,
positive, prednisolone reference group, MA extracts pretreated groups (250–500 mg/kg) for 2 weeks
followed by induction of ulcerative colitis by per-rectal infusion of 8% acetic acid. Macroscopic and
microscopic examinations were done. Infammatory markers (ANCA, CRP and Ilβ6) were measured
in sera. The butanol extracts showed good antioxidant and anti-infammatory activities as they
ameliorated macroscopic and microscopic signs of ulcerative colitis and lowered the infammatory
markers compared to untreated group. MA wastes can be a potential source of bioactive metabolites
for industrial use and future employment as promising anti-ulcerative colitis food supplements.
