Abstract:
This study compares the immunohistochemical reaction of a new experimental
tricalcium silicate perforation repair material to mineral trioxide aggregate (MTA)
and Biodentine. A total of 162 mature premolar teeth from 12 dogs were divided
into three experimental groups (n = 54 teeth each) according to the evaluation
period: 1, 2 and 3 months. Each group was further divided into two equal sub-
groups (n = 27 teeth each) according to the time of repair: immediate repair and
delayed repair. Each subgroup wassubdivided according to the material used into
three experimental subdivisions (n = 8 teeth each): MTA, Biodentine (Septodont)
and experimental material, and two control subdivisions: positive control (n = 2
teeth) and negative control (one tooth). Under general anaesthesia, access cavity
was done. Cleaning and shaping were performed using ProTaper universal rotary
instruments. The canals were obturated using cold lateral compaction technique
with Gutta percha and Adseal sealer. Furcation perforations were created then
randomly sealed using the three materials either immediately or after one month
(delayed repair). Inflammatory cell count and immunohistochemical analysis of
osteopontin-positive area fraction were digitally analysed using the ImageJ soft-
ware. Delayed furcal perforation repair showed significantly higher inflamma-
tory cell count than immediate repair. No significant difference in inflammatory
cell count and immunohistochemical analysis was detected between the three
tested materials. The immunohistochemical analysis revealed the highest im-
munopositive area fraction in the 3-month evaluation period. The experimental
tricalcium silicate cement performed similarly to Biodentine and MTA regarding
the osteopontin expression during perforation repair, suggesting it is a suitable
alternative with favourable handling characters.
