Abstract:
HIAM technique allows the extraction of the original constant signal of each single component out of
interference signals of a mixture and further transformed into basic spectrum (D0
). It includes the meth-
ods: ratio subtraction coupled with unified constant subtraction (RS-UCS), constant center (CC) and con-
stant extraction (CE). The technique was introduced for the analysis of two pharmaceutical formulations
used to treat cardiovascular diseases. The formulations are binary combinations of Amlodipine (AML)
with either Atorvastatin (ATR) or Candesartan (CND) which shows interefernce absorbance signals. The
technique was valid over the linearity range of (5.0–35.0 mg/ml) for AML, ATR and CND with recovery per-
centage 100.40 ± 1.88 , 100.00 ± 0.86 and 99.83 ± 1.07, respectively . The extracted signals were tested for
its purity by spectral contrast angle (cos h) to illustrate the efficency of the HIAM technique where cos h
values ranges from (0.9902 to 0.9986). The presented technique was fully validated regarding ICH guide-
lines and were statistically compared using one-way ANOVA at 95% confidence.