Rapid Screening and Estimation of Binding Constants for Interactions of Fe3+ with Two Metalloproteins, Apotransferrin and Transferrin, Using Affinity Mode of Capillary Electrophoresis
JavaScript is disabled for your browser. Some features of this site may not work without it.
Rapid Screening and Estimation of Binding Constants for Interactions of Fe3+ with Two Metalloproteins, Apotransferrin and Transferrin, Using Affinity Mode of Capillary Electrophoresis
Al Bratty, Mohammed; Alhazmi, Hassan A; Javed, Sadique A; Rehman, Zia Ur; Najmi, Asim; El-Sharkawy, Karam A
e interaction behavior of Fe
3+ with transferrin and apotransferrin (iron-free form) was investigated in
this study using affinity capillary electrophoresis. Change in the mass and charge of protein upon binding to the
metal ion in the capillary tube led to variation in its migration time and was used to measure the noncovalent
binding interactions by fast screening method. Acetanilide was used as the electroosmotic