Abstract:
Ionizing radiation hydrolyzes H2O and leads to free radicals production that initiates inflammation an DNA amages. Siwa ates are an important crop in Egypt that has been use as a
staple foo ue to its nutritional value an its antioxiant properties. The stuy aime to
investigate the raioprotective effects of aqueous extract of Siwa ates in whole boy γ-
irraiate mice. Animals were ivie into four groups: healthy mice, unirraiate mice that
receive Siwa ate aqueous extract, irraiate unprotecte mice an irraiate mice that
receive aily 4 ml/kg Siwa ate extract for 14 ays prior to irraiation. Twenty four hours
post irraiation, bloo an liver samples were collecte for immunological, biochemical, anhistopathological stuies. Apoptosis an apoptotic proteins in hepatocytes were measure by
flow cytometry techniques. Moreover, the cytokinesis-blocke micronucleus assay was performe to measure DNA amages. Matrix metalloproteinases (MMPs) were evaluate in liver
samples by both ELISA an immunohistochemical techniques. The results have shown the
improvement in liver histopathological sections an the amelioration in the pro-inflammatory
cascae. Siwa ate extract allowe DNA protection from the estructive effect of irraiation. In
conclusion, Siwa ates were effective in alleviating raiation inuce amages such as hepatotoxicity, oxiative stress, inflammation, an DNA amage.
