Abstract:
Objective: The objective of this study is to compare and evaluate the antibacterial activity of
modified conventional Glass ionomer restoration (GI) with zinc oxide (ZnO-NPs) and cerium oxide
Nanoparticles (CeO2-NPs) on the oral Streptococcus mutans (SM).
Materials and Methods: one hundred and five disc-shaped specimens were divided into
seven groups; GI, GI incorporated with three, five and seven percent ZnO-NPs and CeO2-NPs.
Antibacterial activity was assessed by counting of colony forming unit (CFU) of SM after direct
contact test and 96 hours biofilm evaluation. Fourier transform infrared spectroscopy (FTIR)
was used to illustrate interaction between different concentrations of Nanoparticles incorporated
with GI.
Results: Direct contact test showed a significant reduction of CFU of GI incorporated with
seven percent ZnO-NPs in relation to GI (P value 0.007). Biofilm evaluation showed significant
reduction of CFU of GI incorporated with three, five and seven percent ZnO-NPs and five and
seven percent CeO2-NPs (P value 0.000). FTIR showed Peaks at 1405 and 1635 cm–1 related to
symmetric and asymmetric vibrations of COO−, those peaks were broader and red shifted in GI
incorporated with ZnO-NPs and CeO-NPs.
Conclusion: Incorporation of conventional GI with ZnO-NPs and CeO2-NPs enhance its
antibacterial activity against oral SM.
