Abstract:
Five methods were developed for simultaneous determination of Salmeterol xinafoate (SAM) and Fluticasone
propionate (FLU) without previous separation. In the first method (HPLC), a reversed-phase column and a mobile
phase of acetonitrile: methanol (80:20 v/v) at 0.5 mLmin-1 flow rate was used to separate both drugs and UV detection
at 220 nm. Linearity was obtained in concentration ranges of 50-500 µgmL-1 for Salmeterol xinafoate Fluticasone
propionate. In the second method both drugs were determined using first derivative UV spectrophotometry, with
zero crossing measurement at 352 and 269.5 nm for Salmeterol xinafoate and Fluticasone propionate, respectively.
The third method depends on first derivative of the ratios spectra by measurements of the amplitudes at 334 and
337.5 nm for Salmeterol xinafoate and at 225 and 231.5 nm for Fluticasone propionate. Calibration graphs were
established in the range of 4-28 µgmL-1 for both Salmeterol xinafoate and Fluticasone propionate. The fourth one
depend on isosbestic point at 237.5 nm, while the content of Salmeterol xinafoate was determined by measuring the
absolute value of the ultraviolet curves at 343 nm, without interference from Fluticasone propionate. All the proposed
methods were extensively validated. They have the advantage of being economic and time saving. All the described
methods can be readily utilized for the analysis of pharmaceutical formulations. The results obtained by adopting the
proposed methods were statistically analyzed and compared with those obtained by official methods.
