The emergence of carbapenemase bla NDM genotype among carbapenem-resistant Enterobacteriaceae isolates from Egyptian cancer patients

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dc.contributor.author Tawfick, M.M
dc.contributor.author Alshareef, W.A
dc.contributor.author Bendary, H.A
dc.contributor.author Elmahalawy, H
dc.contributor.author Abdulall, A.K
dc.date.accessioned 2020-02-28T07:49:39Z
dc.date.available 2020-02-28T07:49:39Z
dc.date.issued 2020-02
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dc.identifier.issn 9349723
dc.identifier.other https://doi.org/10.1007/s10096-020-03839-2
dc.identifier.uri https://t.ly/3GORw
dc.description.abstract Carbapenem resistance among Enterobacteriaceae is a major concern that is increasingly reported worldwide. The objective of this study is to determine the incidence of carbapenem resistance as well as to investigate for carbapenemase-encoding genes among Enterobacteriaceae clinical isolates from cancer patients at different cancer institutes in Egypt. This determination was a cross-sectional study with a total of 135 clinical isolates collected over a period of 1 year. All isolates were sub-cultured on ChromID agar and subjected to phenotypic and molecular detection of carbapenemases. Most of the Enterobacteriaceae isolates were MDR with high resistance rates against tested antimicrobials. Overall, the results of PCR assays revealed that 89.62% (121/135) of isolates harbored one or more of the carbapenemase-encoding genes, while phenotypic assays revealed the production of carbapenemases in 68.88% (93/135) of isolates. BlastN analysis against the non-redundant genome sequences available in the GenBank database revealed that the blaNDM-1 gene was the most prevalent genotype of carbapenemases in 93/135 (68.88%), followed by blaOXA-48 44/135 (32.59%), blaOXA-23 42/135 (31.11%), and blaKPC-2 2/135 (1.48%). Klebsiella pneumoniae isolates harbored the highest number of carbapenemase-encoding genes 34/121 (28.09%). The high prevalence of carbapenemases and/or their encoding genes among MDR Enterobacteriaceae bacteria in Egypt is alarming, thus, the management of serious infections caused by Enterobacteriaceae, particularly in cancer patients will be challenging to clinicians. Carbapenemase blaNDM genotype is emerging in cancer healthcare settings in Egypt, which could be the cause of the current increase in carbapenemase-producing Enterobacteriaceae. © 2020, Springer-Verlag GmbH Germany, part of Springer Nature. en_US
dc.description.uri https://www.scimagojr.com/journalsearch.php?q=19700&tip=sid&clean=0
dc.language.iso en_US en_US
dc.publisher Springer en_US
dc.relation.ispartofseries European Journal of Clinical Microbiology and Infectious Diseases;
dc.subject university of Resistance en_US
dc.subject MDR en_US
dc.subject Enterobacteriaceae en_US
dc.subject Carbapenemases en_US
dc.subject Cancer patients en_US
dc.subject blaNDM en_US
dc.title The emergence of carbapenemase bla NDM genotype among carbapenem-resistant Enterobacteriaceae isolates from Egyptian cancer patients en_US
dc.type Article en_US
dc.identifier.doi https://doi.org/10.1007/s10096-020-03839-2
dc.Affiliation October University for modern sciences and Arts (MSA)


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