Abstract:
Callus and suspension cultures of Iphiona mucronata
grown on Murashige and Skoog medium, supplemented
with 0.1 mg/l kinetin (Kn), 0.1 mg/l naphthalene acetic
acid (NAA) and 5 mg/l ascorbic acid were elicited with
different elicitors. Total flavonoids and phenolics were
determined by aluminum chloride - potassium acetate and
Folin-Ciocalteu colorimetric methods respectively. When
callus and suspension cultures elicited with 10 mg/l yeast
extract (Y), it showed an increase of 2.8 and 3.7 folds for
flavonoids production respectively. When callus cultures
treated with 0.001 mM phenyl alanine (Ph), a slight
increase in flavonoids content reached 1.17 folds, while
treated suspension reached maximum level after 60 hours.
Methyl jasmonate (MJ; 150 µM) increased flavonoids
concentration 4 times for callus and 2.3 times for
suspension. Determination of phenolics for elicited
suspension showed an increase of 2.3 (Y), 2.5 (Ph) and 1.3
(MJ) times. The obtained results favored the use of yeast
extract for high productivity and viability of cell lines.
