Production of flavonoids in callus cultures of Iphiona mucronata, Astraceae

Abstract

Different callus cultures of Iphiona mucronata were established using seedling explants and Murashige & Skoog media (MS) with different phytohormones combinations. The time course for growth of callus culture was followed for the most stable and healthy growing cell lines. Addition of Spirulina platensis extract improved the callus characters but did not increase the growth. The concentration of total flavonoids was calculated by using standard curve for rutin. Total flavonoids produced by callus grown on MS media supplied with 0.1 mg/l kinetin and 0.1 mg/l αnaphthalene acetic acid represents 40% of the plant itself. Addition of 5% Spirolina. platensis extract decreased the productivity of total flavonoids to 21%. Callus grown on MS media supplied with 0.1 mg/l zeatin and 0.1 mg/l α-naphthalene acetic acid produced 24% of total flavonoids compared to the plant itself.