Publisher:Journal of Pharmacognosy and Phytotherapy
Series Info:Journal of Pharmacognosy and Phytotherapy;Volume: 5 Issue: 3 Pages: 47-52
Type:Article
Keywords:University of Tamarix nilotica, cytotoxic activity, sulphorhodamine B (SRB) assay, scavenging activity, 1,1- diphenyl, 2-picrylhydrazyl (DPPH) assay, flavonoid, phenolic.
Abstract:
The radical scavenging activity using 1,1-diphenyl, 2-picrylhydrazyl (DPPH) and cytotoxicity using
sulphorhodamine B (SRB) assay of the aqueous methanolic extract of Tamarix nilotica (Ehrenb.) Bunge
(Tamaricaceae) flowers and its subextracts (CHCl3, EtOAC and Pet.ether) were evaluated. Total phenolic
and flavonoid contents were estimated using colorimetric assays. Ethyl acetate (EtOAc) showed the
highest free radical scavenging activity with inhibitory concentration (IC50) 7.25 ± 0.86 µg/ml in addition
to potential cytotoxic effect on liver cell carcinoma (Huh-7) (IC50 49.1 ± 0.96 µg/ml) whereas effect on
lung cell carcinoma (A-549) was much lower (IC50: 137.9 ± 1.85 µg/ml). EtOAc had the highest flavonoid
content (1.75 ± 1.5 mg/g QE) compared to other subextracts. These results indicated that ethyl acetate
fraction contains bioactive compounds worthy of more sophisticated studies as free radical scavenger
and cytotoxic agent.
