Abstract:
Simple, accurate, sensitive and validated UV stability-indicating spectro- photometric and chemometric methods were developed for determination
of Citalopram Hydrobromide (CT) in presence of its alkaline, oxidative degradation products and in its pharmaceutical preparation. Method (A) is a
successive derivative ratio spectrophotometricone, which depends on the
successive derivative ofratio spectra in two steps and measuring Citalopram
Hydrobromide at 277nm and 293nm. Method (B) is mean centering of ratio
spectra which dependson using the mean centered ratio spectra in two
successive steps and measuring the mean centeredvalues of the second
ratio spectra at 237nm and method (C) used two chemometric techniques ;
principal component regression(PCR) and partial least-squares (PLS). The
proposed methods werechecked using laboratory-prepared mixtures and
were successfully applied for the analysis of pharmaceuticalformulation
containing Citalopram Hydrobromide. The proposed methods were vali- dated according to the ICH guidelines. The obtained results were statisti- cally compared with those obtained froma compendial HPLC method,showing no significant difference with respect to accuracy and precision.
