Abstract:
Two sensitive and precise chromatographic methods were developed and validated for simultaneous
determination of metronidazole (MTR) and diiodohydroxyquin (DIQ) in pharmaceutical preparation. The techniques
adopted for quantification are coupled TLC-densitometry and HPLC. A mixture of chloroform, toluene, ethanol and
acetic acid (9:9:1:1, v/v/v/v) was used as the developing solvent for TLC-densitometry. A mixture of methanol and
acetonitrile, (96:4, v/v) was used as a mobile phase for HPLC at 0.6 mL min-1 flow rate and UV detection at 254
nm. Linearity was obtained in concentration range of 0.5-10 µg spot-1 for DIQ and 1-20 µg spot-1 for MTR applying
TLC-densitometry and 0.005-0.5 mg mL-1 for DIQ and 0.01-0.5 mg mL-1 for MTR applying HPLC. The selectivity of
the proposed methods was checked using laboratory prepared mixtures. The proposed methods were successfully
applied to the analysis of MTR and DIQ in their mixture and in pharmaceutical dosage forms without interference
from other additives.
