Series Info:Pharmacie Globale;Volume: 3 Issue: 5 Pages: 1-4
Type:Article
Keywords:University of metformin; sitagliptin phosphate; spectrophotometry.
Abstract:
Two simple, accurate and precise spectrophotometric methods were developed and validated for the
determination of sitagliptin phosphate monohydrate (STA) and metformin hydrochloride (MTF). The first method
was based on measuring the absorbance of STA at 268 nm in the range of 25-500 µg mL-1. The second method was
the isosbestic point method. The total mixture concentration was calculated by measuring the absorbance at 257
nm. The proposed methods used to determine each drug in binary mixture. The results were statistically
compared using one-way analysis of variance (ANOVA). The developed methods were satisfactory applied to the
analysis of the pharmaceutical formulation and proved to be selective and accurate for the quality control of the
cited drugs in their pharmaceutical formulation.