Evaluating the efficiency of spectral resolution of univariate methods manipulating ratio spectra and comparing to multivariate methods: An application to ternary mixture in common cold preparation
Moustafa A.A.; Salem H.; Hegazy M.; Ali O.
Date issued:
2015
Publisher:
Elsevier B.V.
Series Info:
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
137
Type:
Article
Keywords:
Chlorpheniramine maleate
,
Ibuprofen
,
Multivariate methods
,
Pseudoephedrine HCl
,
Ratio spectra
,
Univariate methods
,
Chlorine compounds
,
Drug products
,
Mixtures
,
Principal component analysis
,
Spectrophotometers
,
Spectrophotometry
,
Chlorpheniramine maleate
,
Ibuprofen
,
Multivariate methods
,
Pseudoephedrine
,
Ratio spectra
,
Univariate method
,
Least squares approximations
,
chlorpheniramine
,
decongestive agent
,
drug combination
,
histamine H1 receptor antagonist
,
ibuprofen
,
nonsteroid antiinflammatory agent
,
pseudoephedrine
,
tablet
,
common cold
,
comparative study
,
drug combination
,
evaluation study
,
limit of detection
,
multivariate analysis
,
procedures
,
regression analysis
,
spectrophotometry
,
tablet
,
Anti-Inflammatory Agents, Non-Steroidal
,
Chlorpheniramine
,
Common Cold
,
Drug Combinations
,
Histamine H1 Antagonists
,
Ibuprofen
,
Least-Squares Analysis
,
Limit of Detection
,
Multivariate Analysis
,
Nasal Decongestants
,
Pseudoephedrine
,
Spectrophotometry
,
Tablets
Abstract:
Simple, accurate, and selective methods have been developed and validated for simultaneous determination of a ternary mixture of Chlorpheniramine maleate (CPM), Pseudoephedrine HCl (PSE) and Ibuprofen (IBF), in tablet dosage form. Four univariate methods manipulating ratio spectra were applied, method A is the double divisor-ratio difference spectrophotometric method (DD-RD). Method B is double divisor-derivative ratio spectrophotometric method (DD-RD). Method C is derivative ratio spectrum-zero crossing method (DRZC), while method D is mean centering of ratio spectra (MCR). Two multivariate methods were also developed and validated, methods E and F are Principal Component Regression (PCR) and Partial Least Squares (PLSs). The proposed methods have the advantage of simultaneous determination of the mentioned drugs without prior separation steps. They were successfully applied to laboratory-prepared mixtures and to commercial pharmaceutical preparation without any interference from additives. The proposed methods were validated according to the ICH guidelines. The obtained results were statistically compared with the official methods where no significant difference was observed regarding both accuracy and precision. � 2014 Elsevier B.V. All rights reserved.
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