A comparative study between three stability indicating spectrophotometric methods for the determination of diatrizoate sodium in presence of its cytotoxic degradation product based on two-wavelength selection
Riad S.M.; El-Rahman M.K.A.; Fawaz E.M.; Shehata M.A.
Date issued:
2015
Publisher:
Elsevier
Series Info:
Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy
145
Type:
Article
Keywords:
October University for Modern Sciences and Arts
,
جامعة أكتوبر للعلوم الحديثة والآداب
,
University of Modern Sciences and Arts
,
MSA University
,
Bivariate
,
Dual wavelength
,
Ratio difference
,
Sodium diatrizoate
,
Stability indicating methods
,
Degradation
,
Photodegradation
,
Sodium
,
Spectrophotometers
,
Bivariate
,
Diatrizoate
,
Dual wavelength
,
Ratio differences
,
Stability indicating method
,
Spectrophotometry
,
diatrizoate
,
meglumine diatrizoate
,
cell death
,
chemistry
,
comparative study
,
drug effects
,
light
,
procedures
,
reproducibility
,
spectrophotometry
,
standard
,
Cell Death
,
Diatrizoate
,
Diatrizoate Meglumine
,
Light
,
Reference Standards
,
Reproducibility of Results
,
Spectrophotometry
Abstract:
Abstract Three sensitive, selective, and precise stability indicating spectrophotometric methods for the determination of the X-ray contrast agent, diatrizoate sodium (DTA) in the presence of its acidic degradation product (highly cytotoxic 3,5-diamino metabolite) and in pharmaceutical formulation, were developed and validated. The first method is ratio difference, the second one is the bivariate method, and the third one is the dual wavelength method. The calibration curves for the three proposed methods are linear over a concentration range of 2-24 ?g/mL. The selectivity of the proposed methods was tested using laboratory prepared mixtures. The proposed methods have been successfully applied to the analysis of DTA in pharmaceutical dosage forms without interference from other dosage form additives. The results were statistically compared with the official US pharmacopeial method. No significant difference for either accuracy or precision was observed. 2015 Elsevier B.V. All rights reserved.
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