Synthesis, characterization, release kinetics and toxicity profile of drug-loaded starch nanoparticles
El-Naggar M.E.; El-Rafie M.H.; El-sheikh M.A.; El-Feky G.S.; Hebeish A.
Date issued:
2015
Publisher:
Elsevier B.V.
Series Info:
International Journal of Biological Macromolecules
81
Type:
Article
Keywords:
Cross-linked starch nanoparticles
,
Drug Delivery
,
Histopathological study
,
In vitro release
,
Nanoprecipitation
,
diclofenac
,
nanoparticle
,
polysorbate 80
,
starch
,
tripolyphosphate
,
drug
,
drug carrier
,
nanoparticle
,
nonsteroid antiinflammatory agent
,
starch
,
animal experiment
,
animal tissue
,
Article
,
controlled study
,
cross linking
,
differential scanning calorimetry
,
encapsulation
,
female
,
histopathology
,
infrared spectroscopy
,
light scattering
,
nonhuman
,
particle size
,
rat
,
skin irritation
,
sustained drug release
,
transdermal drug delivery system
,
transmission electron microscopy
,
X ray diffraction
,
zeta potential
,
animal
,
chemistry
,
cytology
,
drug delivery system
,
drug effects
,
drug formulation
,
drug release
,
kinetics
,
skin
,
ultrastructure
,
Animals
,
Anti-Inflammatory Agents, Non-Steroidal
,
Calorimetry, Differential Scanning
,
Drug Carriers
,
Drug Compounding
,
Drug Delivery Systems
,
Drug Liberation
,
Kinetics
,
Nanoparticles
,
Particle Size
,
Pharmaceutical Preparations
,
Rats
,
Skin
,
Spectroscopy, Fourier Transform Infrared
,
Starch
,
X-Ray Diffraction
Abstract:
The current research work focuses on the medical application of the cost-effective cross-linked starch nanoparticles, for the transdermal delivery using Diclofenac sodium (DS) as a model drug. The prepared DS-cross-linked starch nanoparticles were synthesized using nanoprecipitation technique at different concentrations of sodium tripolyphosphate (STPP) in the presence of Tween 80 as a surfactant. The resultant cross-linked starch nanoparticles loaded with DS were characterized using world-class facilities such as TEM, DLS, FT-IR, XRD, and DSc. The efficiency of DS loading was also evaluated via entrapment efficiency as well as in vitro release and histopathological study on rat skin. The optimum nanoparticles formulation selected by the JMP� software was the formula that composed of 5% maize starch, 57.7mg DS and 0.5% STPP and 0.4% Tween 80, with particle diameter of about 21.04nm, polydispersity index of 0.2 and zeta potential of -35.3mV. It is also worth noting that this selected formula shows an average entrapment efficiency of 95.01 and sustained DS release up to 6h. The histophathological studies using the best formula on rat skin advocate the use of designed transdermal DS loaded cross-linked starch nanoparticles as it is safe and non-irritant to rat skin.The overall results indicate that, the starch nanoparticles could be considered as a good carrier for DS drug regarding the enhancement in its controlled release and successful permeation, thus, offering a promising nanoparticulate system for the transdermal delivery non-steroidal anti-inflammatory drug (NSAID). � 2015 Elsevier B.V.
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