Phenolic profile of centaurea aegyptiaca L. Growing in Egypt and its cytotoxic and antiviral activities
Bakr R.O.; Mohamed S.A.E.H.; Ayoub N.
Date issued:
2016
Publisher:
African Ethnomedicines Network
Series Info:
African Journal of Traditional, Complementary and Alternative Medicines
13
Type:
Article
Keywords:
October University for Modern Sciences and Arts
,
جامعة أكتوبر للعلوم الحديثة والآداب
,
University of Modern Sciences and Arts
,
MSA University
,
Centaurea aegyptiaca
,
Cytotoxicity
,
Flavonoid
,
MTT assay
,
3 (4,5 dimethyl 2 thiazolyl) 2,5 diphenyltetrazolium bromide
,
antineoplastic agent
,
antivirus agent
,
Centaurea aegyptiaca extract
,
Centaurea aegyptiaca plant extract
,
doxorubicin
,
flavone derivative
,
flavonol
,
methanol
,
phenol
,
plant extract
,
unclassified drug
,
antineoplastic agent
,
antivirus agent
,
phenol derivative
,
plant extract
,
antineoplastic activity
,
antiviral activity
,
Article
,
cancer cell line
,
Centaurea
,
Centaurea aegyptiaca
,
chemical composition
,
collisionally activated dissociation
,
controlled study
,
drug mechanism
,
Egypt
,
electrospray mass spectrometry
,
HCT 116 cell line
,
HeLa cell line
,
Hep-G2 cell line
,
Hepatitis A virus
,
Herpes simplex virus 1
,
human
,
human cell
,
IC50
,
liquid chromatography
,
MCF-7 cell line
,
MTT assay
,
nonhuman
,
phytochemistry
,
aerial plant part
,
chemistry
,
tandem mass spectrometry
,
Antineoplastic Agents, Phytogenic
,
Antiviral Agents
,
Centaurea
,
Egypt
,
HCT116 Cells
,
HeLa Cells
,
Hep G2 Cells
,
Humans
,
MCF-7 Cells
,
Phenols
,
Plant Components, Aerial
,
Plant Extracts
,
Tandem Mass Spectrometry
Abstract:
Background: Centaurea aegyptiaca L (Asteraceae), is one of the most attractive plants growing wildly in Sinai, and is not well investigated for its phytochemical constituents. This study represents the first in-depth characterization of the phenolic profile of the aerial parts of C. aegyptiaca methanolic extract utilizing liquid chromatography (LC) combined with electrospray ionization (ESI) tandem mass spectrometry (MS/MS). Material and Methods: Phenolic profile was researched utilizing LC-HRESI-MS-MS. Assessment of cytotoxic activity against four human cancer cell lines (Hep-G2; hepatocellular carcinoma cells, MCF-7; breast adenocarcinoma cells, and HCT-116; colon carcinoma and HELA; cervical carcinoma cells) was performed using 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay. Antiviral activity was surveyed utilizing cytopathic effect inhibition assay. Results: A total of sixty-one compounds were tentatively distinguished (twenty-one phenolic acids and their derivatives, thirty-one flavonols and nine flavones) in the negative and positive modes. Centaurea aegyptiaca demonstrated outstanding results against Hep-G2, MCF-7, HCT-116 and HELA cell lines with IC50of 12.1, 30.9, 11.7 and 19.5 ?g/mL respectively compared and doxorubicin as a reference drug. Weak antiviral activity was seen against hepatitis A virus (HAV) and no impact against herpes simplex virus type 1 (HSV 1). Conclusion: This study provides a better understanding of the chemistry of C. aegyptiaca that announces itself as a promising cytotoxic agent. 2016, African Ethnomedicines Network. All rights reserved.
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