Keywords:bacterial protein
,
clinical trial
,
Egypt
,
female
,
genetics
,
genotyping technique
,
hospital
,
human
,
isolation and purification
,
Klebsiella infection
,
Klebsiella pneumoniae
,
male
,
multicenter study
,
multidrug resistance
,
pathogenicity
,
Bacterial Proteins
,
Drug Resistance, Multiple, Bacterial
,
Egypt
,
Female
,
Genotyping Techniques
,
Hospitals
,
Humans
,
Klebsiella Infections
,
Klebsiella pneumoniae
,
Male
Abstract:
Klebsiella pneumonia infection rates have increased dramatically. Molecular typing and virulence analysis are powerful tools that can shed light on Klebsiella pneumonia infections. Whereas 77.7% (28/36) of clinical isolates indicated multidrug resistant (MDR) patterns, 50% (18/36) indicated carpabenem resistance. Gene prevalence for the AcrAB efflux pump (82.14%) was more than that of the mdtK efflux pump (32.14%) in the MDR isolates. FimH-1 and mrkD genes were prevalent in wound and blood isolates. FimH-1 gene was prevalent in sputum while mrkD gene was prevalent in urine. Serum resistance associated with outer membrane protein coding gene (traT) was found in all blood isolates. IucC, entB, and Irp-1 were detected in 32.14%, 78.5% and 10.7% of MDR isolates, respectively. We used two Polymerase Chain Reaction (PCR) analyses: Enterobacterial Repetitive Intergenic Consensus (ERIC) and Random Amplified Polymorphic DNA (RAPD). ERIC-PCR revealed 21 and RAPD-PCR revealed 18 distinct patterns of isolates with similarity ?80%. ERIC genotyping significantly correlated with resistance patterns and virulence determinants. RAPD genotyping significantly correlated with resistance patterns but not with virulence determinants. Both RAPD and ERIC genotyping methods had no correlation with the capsule types. These findings can help up better predict MDR Klebsiella pneumoniae outbreaks associated with specific genotyping patterns. � The Author(s) 2016.
The incidence of resistance by Enterobacteriaceae to ?-lactam/?- lactamase inhibitors combination is increasing in Egypt. Three phenotypic techniques, comprising AmpC disk diffusion and inhibition dependent methods using ...
Al Yousef S.A.; Younis S.; Farrag E.; Moussa H.S.; Bayoumi F.S.; Ali A.M.(International Journal of Green EconomicsAssociation of Clinical Scientists, 2016)
Background. Urinary tract infection (UTI) is mainly due to invasion of the urethra, bladder or kidneys by pathogens. The emergence of extended spectrum �-lactamases (ESBL) is responsible for frequently observed empirical ...
Tohamy, Sally Tohamy; Aboshanab, Khaled Mohamed; El-Mahallawy, Hadir Ahmed; El-Ansary, Mona R.; Afifi, Salwa Selim(DOVE MEDICAL PRESS LTD, PO BOX 300-008, ALBANY, AUCKLAND 0752, NEW ZEALAND, 2018)
Introduction: Bloodstream infections with multidrug-resistant (MDR) Gram-negative bacteria (GNB) are among the most frequent complications in immunocompromised cancer patients because of their considerable morbidity and ...