Carriage frequency, phenotypic, and genotypic characteristics of methicillin-resistant Staphylococcus aureus isolated from dental health-care personnel, patients, and environment
Khairalla A.S.; Wasfi R.; Ashour H.M.
Date issued:
2017
Publisher:
Life Science Journal
,
Nature Publishing Group
Series Info:
Scientific Reports
7
Type:
Article
Keywords:
October University for Modern Sciences and Arts
,
جامعة أكتوبر للعلوم الحديثة والآداب
,
University of Modern Sciences and Arts
,
MSA University
,
bacterial protein
,
biofilm
,
classification
,
cross-sectional study
,
dental clinic
,
Egypt
,
environmental microbiology
,
genetics
,
growth, development and aging
,
hand
,
health care personnel
,
heterozygote
,
human
,
isolation and purification
,
methicillin resistant Staphylococcus aureus
,
microbial sensitivity test
,
microbiology
,
molecular evolution
,
molecular typing
,
nose
,
phylogeny
,
procedures
,
Staphylococcus infection
,
tooth disease
,
transmission
,
Bacterial Proteins
,
Biofilms
,
Carrier State
,
Cross-Sectional Studies
,
Dental Clinics
,
Egypt
,
Environmental Microbiology
,
Evolution, Molecular
,
Hand
,
Health Personnel
,
Humans
,
Methicillin-Resistant Staphylococcus aureus
,
Microbial Sensitivity Tests
,
Molecular Typing
,
Nose
,
Phylogeny
,
Staphylococcal Infections
,
Tooth Diseases
Abstract:
There is limited data on methicillin-resistant Staphylococcus aureus (MRSA) carriage in dental clinics.1300 specimens from patients, health personnel, and environmental surfaces of a dental clinic in Egypt were tested for MRSA.Antibiotic susceptibility, biofilm formation, Staphylococcal protein A (spa) typing, SCCmec typing, and PCR-based assays were used to detect mecA, mecC, vanA, Panton-Valentine Leukocidin toxin (PVL), and toxic shock syndrome toxin-1 (tst) genes.Among 34 mecA-positive MRSA isolates, five (14.7%) were PVL-positive, seventeen (50%) were tst-positive, ten (29.4%) were vanA-positive, while none harboured mecC.MRSA hand carriage rates in patients, nurses, and dentists were 9.8%, 6.6%, and 5%.The respective nasal colonization rates were 11.1%, 6.7%, and 9.7%.1.3% of the environmental isolates were MRSA-positive.Strong and moderate biofilm-forming isolates represented 23.5% and 29.4% of MRSA isolates.24 MRSA isolates (70.6%) were multi-resistant and 18 (52.9%) harboured SCCmec IV.Among eight spa types, t223 (26.5%), t267 (23.5%), and t14339 (23.5%) were predominant.We noted an alarming genetic relatedness between 7 (20.6%) MRSA isolates and the epidemic EMRSA-15 clone, as well as a combined occurrence of tst and PVL in 3 (8.8%) isolates.Results suggest high MRSA pathogenicity in dental wards highlighting the need for more efficient surveillance/infection control strategies. � 2017 The Author(s).
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