Novel contribution to the simultaneous monitoring of pramipexole dihydrochloride monohydrate and levodopa as co-administered drugs in human plasma utilizing UPLC-MS/MS
Mohamed D.; Hegazy M.A.; Elshahed M.S.; Toubar S.S.; Helmy M.I.
Date issued:
2018
Publisher:
SAGE Publications Ltd
Series Info:
European Journal of Mass Spectrometry
24
Type:
Article
Keywords:
diphenhydramine
,
healthy volunteers
,
levodopa
,
Pramipexole dihydrochloridemonohydrate
,
ultra-performance liquid chromatography tandem mass spectrometry
,
Liquid chromatography
,
Mass spectrometry
,
Plasma (human)
,
Diphenhydramine
,
Healthy volunteers
,
Levodopa
,
Pramipexole
,
Ultra performance liquid chromatography
,
Drug products
,
dopamine receptor stimulating agent
,
levodopa
,
pramipexole
,
blood
,
chemistry
,
drug stability
,
evaluation study
,
high performance liquid chromatography
,
human
,
intravenous drug administration
,
procedures
,
tandem mass spectrometry
,
Administration, Intravenous
,
Chromatography, High Pressure Liquid
,
Dopamine Agonists
,
Drug Stability
,
Humans
,
Levodopa
,
Pramipexole
,
Tandem Mass Spectrometry
Abstract:
An efficient, selective, sensitive, and rapid ultra-performance liquid chromatography tandem mass spectrometry method was established and validated for the quantification of pramipexole dihydrochloride monohydrate and levodopa simultaneously in human plasma with the aid of diphenhydramine as an internal standard. A simple protein precipitation technique with HPLC grade acetonitrile was efficiently utilized for the cleanup of plasma. The analysis was performed using a Hypersil gold 50 mm � 2.1 mm (1.9 �m) column and a mobile phase of 0.2% formic acid and methanol (90: 10 v/v). The triple-quadrupole mass spectrometer equipped with an electrospray source operated in the positive mode was set up in the selective reaction monitoring mode (SRM) to detect the ion transitions m/z 212.15 ?153.01, m/z 198.10? 135.16, and m/z 255.75 ? 166.16 for pramipexole dihydrochloride monohydrate, levodopa, and diphenhydramine, respectively. The method was thoroughly validated according to FDA guidelines and proved to be linear, accurate, and precise over the range 100-4000 pg/mL for pramipexole dihydrochloride monohydrate and 60-4000 ng/mL for levodopa. The proposed method was effectively applied for monitoring both drugs in plasma samples of healthy volunteers. The Author(s) 2018.
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